Figure 6
From: Molecular cloning and characterization of Izumo1 gene from bovine testis
Dimeric formation of bovine IZUMO1. (A) Protein extracts from bovine sperm, were separated by SDS-PAGE under non-reducing conditions and subjected to Western blot analysis using an antibody raised against bIZUMO1. Arrows and asterisks indicate the bIZUMO1 complex and monomeric forms, respectively. (B) Biotinylation assay of bovine sperm surface molecles: (+) biotin-labeled sperm extracts and (−) non-botinylated sperm extracts.