Primer Name | Sequence (5’-3’) | Fragment size (bp) | T.M. (°C) |
---|---|---|---|
Cloning primers | |||
TRα-1 F | CCTGGATGGAATTGAAGTGA | 799 | 62.0 |
TRα-1R | GACATGATCTCCATGCAGC | ||
TRα-2 F | AGGCCTTCAGCGAGTTTAC | 652 | 59.0 |
TRα-2R | CCTTCTCTCCAGGCTCCTC | ||
CRABPII-1 F | CAGTGCTCCAGTGGAAAGA | 563 | 56.5 |
CRABPII-1R | CCAGAAGTGATTGGGTGAG | ||
Real-time PCR primers | |||
TRα-3 F | TTACCTGGACAAAGACGAGC | 113 | 57.4 |
TRα-3R | TCTGGATTGTGCGGCGAAAG | ||
CRABPII-2 F | ACATCAAAACCTCCACCACC | 111 | 56.5 |
CRABPII-2R | CCCATTTCACCAGGCTCTTA | ||
ACTB-F | CCTGCGGCATTCACGAAACTAC | 87 | 58.5 |
ACTB-R | ACAGCACCGTGTTGGCGTAGAG | ||
GAPDH-F | GCA AGTTCCACGGCACAG | 249 | 59.0 |
GAPDH-R | GGT TCACGCCCATCACAA | ||
TOP2B-F | GTGTGGAGCCTGAGTGGTATA | 137 | 59.0 |
TOP2B-R | AAGCATTCGCCTGACATTGTT |