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Table 1 Composition of IVD 101 medium and IVMD 101 mediuma

From: Effects of culture media conditions on production of eggs fertilized in vitro of embryos derived from ovary of high grade Hanwoo

Components

IVD 101

IVMD 101

D-glucose(mM)

2.22

5.56

Sodium pyruvate(mM)

0.27

0.91

Sodium lactate(mM)

2.48

 

L-cysteine(mM)

0.05

 

GSH(μm)

200

 

Taurine(mM)

5

5

Selenium(mM)

5

5

Insulin(ug/Μℓ)

 

5

TGF-a(mg/Μℓ)

 

10

Apo-transferrin(ug/Μℓ)

10

10

bFGF(mg/Μℓ)

10

 

TGF-B1(mg/Μℓ)

1

 

TIMP-1(ug/Μℓ)

0.5

 

Aprotinin(ug/Μℓ)

0.5

 

BSA(mg/Μℓ)

1

1

HEPES(mM)

5

5

Gentamycin sulfate(ug/Μℓ)

10

10

  1. aBasal medium of IVD 101 and IVMD 101