Acute phase protein mRNA expressions and enhancement of antioxidant defense system in Black-meated Silkie Fowls supplemented with clove (Eugenia caryophyllus) extracts under the influence of chronic heat stress

Table 4 Effect of elevated temperature and clove supplementation on immunological organs of black-meated fowls from 42 to 63 d^a

Treatment		Immune organ growth index (mg/g)
Treatment	Liver (g/bird)	Spleen	Bursa of Fabricius	Thymus
TC,^b added clove, mg/kg
0	32.68	1.30	3.99	1.13
ET,^b added clove, mg/kg
0	27.87	1.18	3.53	0.90
200	29.72	1.34	4.32	1.00
400	33.01	1.51	4.68	1.12
600	35.34	1.55	5.66	1.17
SEM	0.68	0.04	0.13	0.03
	P-value
TC vs. ET at 0 mg of clove/kg	0.043	0.016	0.140	0.003
Effects of clove under ET
Linear	0.004	0.014	0.039	0.025
Quadratic	0.093	0.148	0.341	0.007

^aData are means of 2 birds per replicate (5 birds per cage; 7 replicates per group). ^bTC: Thermoneutral temperature treatment (24 ± 2 °C); ET: Elevated temperature treatment (35 ± 2 °C)

ISSN: 2055-0391