In vitro rumen fermentation kinetics, metabolite production, methane and substrate degradability of polyphenol rich plant leaves and their component complete feed blocks

Table 2 In vitro fermentation constants, degradability and methane production of different forages

Attributes	Vigna sinensis Hay	Acacia nilotica leaves	Ziziphus nummularia leaves	SEM	Significance (P value)
Fermentation kinetics
Potential gas production (ml/200 mg DM)	42.1	40.9	37.9	0.926	0.021
Rate constant (c)	0.044	0.061	0.049	0.005	0.001
Half time (t^1/2, h)	15.6	11.4	14.0	0.71	0.018
Lag phase(L, h)	1.90	3.33	5.37	1.16	0.012
Degradability and methane production
DMD %	57.7^b	65.0^a	54.9^c	0.31	< 0.001
OMD %	64.6^b	68.8^a	60.2^c	0.59	< 0.001
Gas production (mL/g)	137^b	151^a	126^c	3.1	< 0.001
Gas production (mL/g DDM)	237^a	232^ab	229^b	2.5	0.024
Gas production (mL/g DOM)	240	250	239	5.2	0.541
Methane production (mL/g DM)	28.4^a	12.7^c	23.0^b	0.95	< 0.001
Methane production (mL/g DDM)	49.1^a	19.6^c	41.9^b	1.36	< 0.001
Methane production (mL/g OMD)	49.8^a	22.3^c	43.8^b	0.70	< 0.001
Partitioning factor (g/mL)	4.16^b	3.99^a	4.18^b	0.038	0.042

Means bearing different superscript (^{a, b, c}) differ significantly
μm = maximum rate of GP (b × c) At the inflection point (Y = b/2), i.e. inflection occurs halfway to the maximum gas volume and thus, t_1/2 = L + (μm × b/2)

ISSN: 2055-0391