Skip to main content

Advertisement

Table 2 List of insulin signaling pathway primers for qPCR

From: Changes in expression of insulin signaling pathway genes by dietary fat source in growing-finishing pigs

Gene Symbol Primer sequences Forward / Reverse Product Size (bp)
INSR F:5′-TTCACTGGCAATCGCATTGAGCTG-3′ 137 bp
  R:5′-TCATGGGTCACAGGGCCAATGATA-3′  
IRS F:5′-AGGAAGTTTGGCAGGTGATCCTGA-3′ 200 bp
  R:5′-ACGGCCCACTTCGATGAAGAAGAA-3′  
PIP3 F:5′-CTTTGCAGAGCTTGACCCAGAT-3′ 100 bp
  R:5′-GAGCTTGTGGGCTTGCCTTCATTT-3′  
PDK1 F:5′-GGAAACCCTTGGCACCAGTTTGTA-3′ 183 bp
  R:5′-TCGGAGTTCTTGTGACCACGGAAT-3′  
Akt F:5′-AGAAGCTCTTCGAGCTCATCCTCA-3′ 148 bp
  R:5′-TGCATGATCTCCTTGGCATCCTCA-3′  
FOXO1 F:5′-TCCCACACAGTGTCAAGACAACGA-3′ 118 bp
  R:5′-ACTGCTTCTCTCAGTTCCTGCTGT-3′  
PDE3 F:5′-CCTGCAGAACCACAAGATGTGGAA-3′ 190 bp
  R:5′-TCACTGGTTTGGCTTTGGTGTTGG-3′  
  1. INSR, insulin receptor; IRS, insulin receptor substrate; PIP3, phosphatidylinositol 3-kinase; PDK1, 3-phosphoinositide-dependent protein kinase-1; Akt, protein kinase B; FOXO1, forkhead box protein O1; PDE3, cGMP-inhibited 3’,5’-cyclic phosphodiesterase.