Table 2 List of insulin signaling pathway primers for qPCR
Gene Symbol | Primer sequences Forward / Reverse | Product Size (bp) |
|---|---|---|
INSR | F:5′-TTCACTGGCAATCGCATTGAGCTG-3′ | 137 bp |
R:5′-TCATGGGTCACAGGGCCAATGATA-3′ | ||
IRS | F:5′-AGGAAGTTTGGCAGGTGATCCTGA-3′ | 200 bp |
R:5′-ACGGCCCACTTCGATGAAGAAGAA-3′ | ||
PIP3 | F:5′-CTTTGCAGAGCTTGACCCAGAT-3′ | 100 bp |
R:5′-GAGCTTGTGGGCTTGCCTTCATTT-3′ | ||
PDK1 | F:5′-GGAAACCCTTGGCACCAGTTTGTA-3′ | 183 bp |
R:5′-TCGGAGTTCTTGTGACCACGGAAT-3′ | ||
Akt | F:5′-AGAAGCTCTTCGAGCTCATCCTCA-3′ | 148 bp |
R:5′-TGCATGATCTCCTTGGCATCCTCA-3′ | ||
FOXO1 | F:5′-TCCCACACAGTGTCAAGACAACGA-3′ | 118 bp |
R:5′-ACTGCTTCTCTCAGTTCCTGCTGT-3′ | ||
PDE3 | F:5′-CCTGCAGAACCACAAGATGTGGAA-3′ | 190 bp |
R:5′-TCACTGGTTTGGCTTTGGTGTTGG-3′ |