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Table 1 The primer sequence used to amplify the exonic sequence variants in the bovine FTO gene

From: Novel SNP in the coding region of the FTO gene is associated with marbling score in Hanwoo (Korean cattle)

Primer Primer sequences (5′-3′) Amplified region Fragment size (bp) Location GenBank accession no.
FTO-1 F-GGCTATAACGGCAGCATGAA exon 1 844 4 ~ 847 AC_000175.1
R-CCAGGTGGTGAAGATGGAAA
FTO-2 F-AAGGCATTGTCAGCCTGGATG exon 2 714 107821 ~ 108536
R-ATATGGTACAAGGAGCACCAGG
FTO-3 F-TGTCTGTAATGGAGCTTGGACC exon 3 906 124883 ~ 125788
R-CAATCATATCTCCAGAGCCTGC
FTO-4 F-CCTTTGCAACTCTCTAGTCCTGTG exon 4 1064 144220 ~ 145283
R-CTCTCCAAACTCCCCAAACTTC
FTO-5 F-TTTCCAGGCAAGAGTACTGGAG exon 5 675 166054 ~ 166728
R-CTTGACCTTGACCTTGACCTTG
FTO-6 F-AACGTGGGGTTCACTTACTGTG exon 6 651 175553 ~ 176203
R-GCAAGAATACTGGAGTGGGTTG
FTO-7 F-CTGTCATGTGCTTTAGGATCTC exon 7 617 183602 ~ 184218
R-TGTCTGTGAGTCAACTTCTGTT
FTO-8 F-TAAGGCAGTGGAGCTTTGGA exon 8 821 235720 ~ 236540
R-TGTGCACAGAAATGAGGCTG
FTO-9 F-TCACAGTGGATTGCCAAGGT exon 9 824 420877 ~ 421700
R-TGTGGCTCATGCAACTGAAG