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Table 3 Effects of dietary supplementations of native ZnO and lipid-encapsulated ZnO (LE ZnO) on hepatic and circulating Zn concentrations, intestinal villus structure, and specific activities of digestive enzymes in weanling pigs

From: Effects of a lipid-encapsulated zinc oxide supplement on growth performance and intestinal morphology and digestive enzyme activities in weanling pigs

Enzyme     Dietary supplementation (ppm as Zn) Contrast P-value
  Native ZnO1) LE ZnO P-value LE ZnO vs. Within LE ZnO
  100 2,500 SEM 8% lipid2) 10% lipid2) 12% lipid2) SEM   Native ZnO 10% lipid vs. 100 vs.
     100 250 100 250 100 250    100 2,500 8% 12% 250
Zn concentration                 
  Liver (μg/g) 34.9 412.1 13.6 33.3 55.4 27.8 33.5 36.3 39.0 19.2 <0.01 0.87 <0.01 0.48 0.72 0.52
 Serum (μg/ml) 1.78 2.45 0.14 1.53 1.63 1.52 1.50 1.14 1.62 0.22 <0.01 0.10 <0.01 0.75 0.57 0.31
Villus structure               
 Duodenum                 
   VH (μm) 292 284 14 299 365 299 315 277 323 20 0.07 0.21 0.10 0.22 0.71 <0.01
   CD(μm) 230 227 11 240 282 238 276 231 256 16 0.07 0.07 0.05 0.78 0.41 <0.01
   VH:CD 1.27 1.26 0.04 1.25 1.29 1.26 1.15 1.20 1.26 0.05 0.56 0.37 0.66 0.21 0.60 0.98
 Jejunum                 
   VH (μm) 280 252 16 254 315 240 266 275 263 21 0.31 0.54 0.38 0.15 0.46 0.17
  CD(μm) 230 206 15 234 262 225 238 251 218 20 0.49 0.64 0.08 0.44 0.89 0.87
   VH:CD 1.21 1.16 0.03 1.09 1.20 1.07 1.12 1.11 1.20 0.04 0.03 0.02 0.30 0.21 0.13 <0.01
  Ileum                 
   VH (μm) 245 209 13 215 247 245 234 230 232 19 0.56 0.48 0.11 0.67 0.67 0.62
   CD(μm) 228 186 13 205 221 238 224 238 180 19 0.15 0.73 0.05 0.33 0.24 0.24
   VH:CD 1.10 1.21 0.07 1.30 1.16 1.03 1.05 1.09 1.31 0.11 0.36 0.54 0.50 0.08 0.15 0.71
Enzyme activity (μmol end product · mg protein-1 · min-1)            
  Sucrase                 
  Duodenum 0.043 0.034 0.008 0.076 0.039 0.040 0.049 0.041 0.044 0.012 0.25 0.61 0.14 0.26 0.89 0.39
  Jejunum 0.874 0.537 0.225 0.319 0.401 0.160 0.628 0.509 0.170 0.318 0.59 0.06 0.51 0.91 0.86 0.79
   Ileum 0.098 0.117 0.032 0.105 0.099 0.088 0.107 0.094 0.119 0.046 1.00 0.91 0.68 0.93 0.86 0.74
  Maltase                 
  Duodenum 5.18 4.41 1.10 8.15 4.56 5.98 7.71 5.34 7.80 1.56 0.36 0.28 0.10 0.76 0.86 0.88
  Jejunum 15.87 12.70 3.48 8.35 12.95 10.28 14.10 13.88 5.38 4.92 0.76 0.22 0.64 0.76 0.61 0.99
  Ileum 4.20 5.39 1.49 5.11 4.02 2.91 6.27 4.13 3.95 2.11 0.96 0.91 0.57 0.99 0.80 0.69
  LAP                 
  Duodenum 2.00 2.30 0.36 2.19 1.91 1.99 2.72 1.90 3.20 0.50 0.53 0.45 0.96 0.55 0.70 0.17
  Jejunum 3.15 2.73 0.27 2.37 2.53 3.17 2.51 3.25 2.20 0.38 0.30 0.14 0.86 0.31 0.77 0.11
  Ileum 1.76 1.93 0.29 2.04 1.71 1.68 1.94 1.61 1.81 0.41 0.99 0.90 0.69 0.88 0.81 0.89
 Amylase (PAN) 241.2 142.1 23.0 262.1 185.1 224.3 240.7 237.6 231.4 31.4 0.06 0.67 <0.01 0.78 0.95 0.39
 Trypsin (PAN) 0.156 0.107 0.022 0.149 0.127 0.150 0.184 0.160 0.197 0.032 0.43 0.86 0.06 0.37 0.71 0.53
  1. 1)Data are means of 8 animals in each group.
  2. 2)Denotes the percentage of lipid (w/w) encapsulating the ZnO particle. Data are means of 4 animals in each group.
  3. VH, villus height; CD, crypt depth; LAP, leucine aminopeptidase; PAN, pancreas.