The effect of 1, 25 (OH)
on protein expression of Sox 9 in equine articular chondrocytes following 24 h of incubation. (A) Western blot analysis of protein expression levels using an antibody raised against the Sox 9 protein. Freshly isolated equine whole chondrocyte lysate was used as a positive control C. β-actin was used as an internal load control. (B) the level of expression of the protein following 24 h of incubation with different doses (1 × 10−9 and 1 × 10−12) of 1, 25 (OH)2D3 treatment in addition to the solvent (DMSO). Values are presented as means ± S.E. ***Indicates statistically significant at P < 0.001.