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Figure 1 | Journal of Animal Science and Technology

Figure 1

From: Influence of 1α, 25-dihydroxyvitamin D3 [1, 25(OH)2D3] on the expression of Sox 9 and the transient receptor potential vanilloid 5/6 ion channels in equine articular chondrocytes

Figure 1

The effect of 1, 25 (OH) 2 D 3 on protein expression of Sox 9 in equine articular chondrocytes following 24 h of incubation. (A) Western blot analysis of protein expression levels using an antibody raised against the Sox 9 protein. Freshly isolated equine whole chondrocyte lysate was used as a positive control C. β-actin was used as an internal load control. (B) the level of expression of the protein following 24 h of incubation with different doses (1 × 10−9 and 1 × 10−12) of 1, 25 (OH)2D3 treatment in addition to the solvent (DMSO). Values are presented as means ± S.E. ***Indicates statistically significant at P < 0.001.

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