Expression of TRPV5/6 at the mRNA level. End point PCR analysis of TRPV5 (A) and TRPV6 (B) ion channel genes on mRNA isolated from equine articular chondrocytes. The graphs show real-time PCR analysis of TRPV5 (C) and TRPV6 (D) ion channel gene expression levels. Experiments were carried out on equine articular chondrocytes (control) and treated with different doses of 1, 25 (OH)2D3. GAPDH and HPRT were included as internal controls. Data presented as a mean ± S.E.