Table 1 Primer information for 30 microsatellite markers used in this study*
From: Discrimination of the commercial Korean native chicken population using microsatellite markers
Marker | Chr. | Dye | Forward (5′ → 3′) | Reverse (5′ → 3′) | Allele size (bp) |
|---|---|---|---|---|---|
ADL0268 | 1 | PET | CTCCACCCCTCTCAGAACTA | CAACTTCCCATCTACCTACT | 105-117 |
MCW0111 | 1 | NED | GCTCCATGTGAAGTGGTTTA | ATGTCCACTTGTCAATGATG | 98-112 |
MCW0145 | 1 | FAM | ACTTTATTCTCCAAATTTGGCT | AAACACAATGGCAACGGAAAC | 181-211 |
MCW0063 | 2 | FAM | GGCTCCAAAAGCTTGTTCTTAGCT | GAAAACCAGTAAAGCTTCTTAC | 132-150 |
MCW0087 | 2 | NED | ATTTCTGCAGCCAACTTGGAG | CTCAGGCAGTTCTCAAGAACA | 267-283 |
LEI0141 | 2 | FAM | CGCATTTGATGCATAACACATG | AAGGCAAACTCAGCTGGAACG | 220-242 |
MCW0039 | 2 | VIC | CATTGGACTGAGATGTCACTGCAG | ACATTTGTCTAATGGTACTGTTAC | 127-147 |
MCW0264 | 2 | FAM | CTTACTTTTCACGACAGAAGC | AGACTGAGTCACACTCGTAAG | 224-240 |
MCW0288 | 2 | FAM | GATCTGCTTCTCTGCCCCATG | GGTACTGTCACCAGAATGAGC | 108-122 |
MCW0127 | 3 | VIC | GAGTTCAGCAGGAATGGGATG | TGCAATAAGAGAAGGTAAGGTC | 227-241 |
MCW0040 | 3 | VIC | ACTCAAAAATGTGGTAGAATATAG | ACCGAAATTGAGCAGAAGTTA | 121-145 |
ADL0317 | 4 | FAM | AGTTGGTTTCAGCCATCCAT | CCCAGAGCACACTGTCACTG | 178-204 |
LEI0094 | 4 | FAM | GATCTCACCAGTATGAGCTGC | TCTCACACTGTAACACAGTGC | 254-280 |
ADL0292 | 5 | FAM | CCAAATCAGGCAAAACTTCT | AAATGGCCTAAGGATGAGGA | 110-138 |
MCW0029 | 5 | VIC | GTGGACACCCATTTGTACCCTATG | CATGCAATTCAGGACCGTGCA | 139-189 |
ROS0013 | 5 | NED | TGCTGCTCCTGGRAAATTG | GAAAAGCCATGGAGGAATCA | 220-242 |
ADL0159 | 6 | VIC | GCCATTATTTTTCCCTGTGT | CTCCCCAAAGTCATTAGCAG | 107-127 |
ROS0019 | 7 | NED | ATGTACAGGTTCCAGTGTCCG | CCAGTTCATACAACCTTGAGTTGG | 119-143 |
ADL0259 | 9 | VIC | CTCATTGCAGAGGAAGTTCT | GTAATGGAGGATGCTCAGGT | 107-129 |
GCT0016 | 9 | NED | TCCAAGGTTCTCCAGTTC | GGCATAAGGATAGCAACAG | 109-125 |
MCW0228 | 10 | PET | GATCTCTGCATTACAAGCATG | TTGCTGACCTGCTCATGCAAG | 221-239 |
MCW0104 | 13 | FAM | TAGCACAACTCAAGCTGTGAG | AGACTTGCACAGCTGTGTACC | 189-225 |
ROS0083 | 13 | VIC | CATTACAGCTCAGTGTTGGCA | TTGCAAGTGCTCTCCCATC | 109-129 |
MCW0213 | 13 | NED | GACAAGTCAACAACTTGCCAG | CTGTTCACTTTAAGGACATGG | 288-316 |
MCW0123 | 14 | FAM | CCACTAGAAAAGAACATCCTC | GGCTGATGTAAGAAGGGATGA | 79-89 |
ADL0293 | 17 | PET | GTAATCTAGAAACCCCATCT | ACATACCGCAGTCTTTGTTC | 105-119 |
MCW0330 | 17 | VIC | TGGACCTCATCAGTCTGACAG | AATGTTCTCATAGAGTTCCTGC | 254-286 |
ADL0304 | 18 | FAM | GGGGAGGAACTCTGGAAATG | CCTCATGCTTCGTGCTTTTT | 137-159 |
LEI0074 | 26 | VIC | GACCTGGTCCTGACATGGGTG | GTTTGCTGATTAGCCATCGCG | 224-240 |
LEI0135 | 28 | NED | CACAATGAAGGATGAATAGTGC | AATTCACAGTTACACCTGAGG | 131-142 |