Effect of dietary supplementation with Spirulina on the expressions of AANAT , ADRB3 , BTG2 and FASN genes in the subcutaneous adipose and Longissimus dorsi muscle tissues of purebred and crossbred Australian sheep

Table 1 Primer pairs designed for real-time PCR (qPCR)

^a Gene symbol	qPCR primers		^b T _a	Amplicon size (bp)
	Forward Primer	Reverse Primer
AANAT	ACTGACCTTCACGGAGATGC	TTCACTCATTCTCCCCGTTC	60	211
ADRB3	TCAGTAGGAAGCGGGTCGGG	GGCTGGGGAAGGGCAGAGTT	60	291
BTG2	CTGGAGGAGAACTGGCTGTC	AAAACAATGCCCAAGGTCTG	60	194
FASN	GTGTGGTACAGCCCCTCAAG	ACGCACCTGAATGACCACTT	60	110
Reference genes
UBC	CGTCTTAGGGGTGGCTGTTA	AAATTGGGGTAAATGGCTAGA	60	90
PPIA	TCATTTGCACTGCCAAGACTG	TCATGCCCTCTTTCACTTTGC	60	72

^aAralkylamine N-acetyltransferase = AANAT, β3-adrenergic receptor = ADRB3, B-cell translocation gene 2 = BTG2, Fatty acid synthase = FASN, Ubiquitin C = UBC, Peptidyl-prolyl cis-trans isomeraseA = PPIA, ^bTa = Empirical annealing Temperature.