|
a
Gene symbol
|
qPCR primers
|
b
T
a
|
Amplicon size (bp)
|
|---|
| |
Forward Primer
|
Reverse Primer
| | |
|
AANAT
|
ACTGACCTTCACGGAGATGC
|
TTCACTCATTCTCCCCGTTC
|
60
|
211
|
|
ADRB3
|
TCAGTAGGAAGCGGGTCGGG
|
GGCTGGGGAAGGGCAGAGTT
|
60
|
291
|
|
BTG2
|
CTGGAGGAGAACTGGCTGTC
|
AAAACAATGCCCAAGGTCTG
|
60
|
194
|
|
FASN
|
GTGTGGTACAGCCCCTCAAG
|
ACGCACCTGAATGACCACTT
|
60
|
110
|
|
Reference genes
| | | | |
|
UBC
|
CGTCTTAGGGGTGGCTGTTA
|
AAATTGGGGTAAATGGCTAGA
|
60
|
90
|
|
PPIA
|
TCATTTGCACTGCCAAGACTG
|
TCATGCCCTCTTTCACTTTGC
|
60
|
72
|
-
aAralkylamine N-acetyltransferase = AANAT, β3-adrenergic receptor = ADRB3, B-cell translocation gene 2 = BTG2, Fatty acid synthase = FASN, Ubiquitin C = UBC, Peptidyl-prolyl cis-trans isomeraseA = PPIA, bTa = Empirical annealing Temperature.
