Figure 2

Derivative melting-curve analysis using SYBR Green to identify the dissociation temperature of reference gene amplicons and the specificity of RT-PCR reactions using total RNA from equine marrow- and adipose derived MSCs. The Optical System Software plotted the rate of change of the relative fluorescence units with time (dF)/dT) on the Y-axis versus the temperature (°C) on the X-axis, which peaks at the melting temperature (Tm). Similar peaks indicate no contamination and primer–dimer artifact. BM = Bone marrow; AT = Adipose tissue; MSCs = Mesenchymal stem cells.