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Figure 2 | Journal of Animal Science and Technology

Figure 2

From: GAPDH, β-actin and β2-microglobulin, as three common reference genes, are not reliable for gene expression studies in equine adipose- and marrow-derived mesenchymal stem cells

Figure 2

Derivative melting-curve analysis using SYBR Green to identify the dissociation temperature of reference gene amplicons and the specificity of RT-PCR reactions using total RNA from equine marrow- and adipose derived MSCs. The Optical System Software plotted the rate of change of the relative fluorescence units with time (dF)/dT) on the Y-axis versus the temperature (°C) on the X-axis, which peaks at the melting temperature (Tm). Similar peaks indicate no contamination and primer–dimer artifact. BM = Bone marrow; AT = Adipose tissue; MSCs = Mesenchymal stem cells.

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