Table 1 Information of evaluated internal control genes and their primer sequences
Reference gene | Accession number | function | Forward primer | Reverse primer | Amplicon size (bp) |
|---|---|---|---|---|---|
Glyceraldehyde-3- phosphate dehydrogenase (GAPDH) | NM_001163856 | Oxidoreductase in glycolysis and gluconeogenesis | TGTCATCAACGGAAAGGC | GCATCAGCAGAAGGAGCA | 183 |
β-actin | NM_001081838 | Cytoskeletal structural protein | GGGCATCCTGACCCTCAAG | TCCATGTCGTCCCAGTTGGT | 63 |
Β2-microglobulin (B2M) | NM_001082502 | A component of MHC class I molecule | CGAAGGTTCAGGTTTACTCACG | ATTTCAATCTCAGGCGGA | 97 |