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Table 2 Threshold cycles (C T ) of GAPDH, β-actin and B2M in equine bone marrow- and adipose tissue- derived MSCs

From: GAPDH, β-actin and β2-microglobulin, as three common reference genes, are not reliable for gene expression studies in equine adipose- and marrow-derived mesenchymal stem cells

Gene Case N. Replicates BM-MSCs AT-MSCs
    C T mean SD C T mean SD
GAPDH 3 3 19.11a 2.52 16.19b 0.49
ACTB 3 3 21.97a 2.21 18.05b 0.70
B2M 3 3 22.35a 3.14 17.66b 1.09
  1. Letters (a, b) represent significant differences in expression levels of GAPDH (P < 0.05), β-actin (P < 0.001) and B2M (P < 0.006) between BM- and AT-derived MSCs. BM = Bone marrow; AT = Adipose tissue; MSCs = Mesenchymal stem cells.