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Table 2 Threshold cycles (C T ) of GAPDH, β-actin and B2M in equine bone marrow- and adipose tissue- derived MSCs

From: GAPDH, β-actin and β2-microglobulin, as three common reference genes, are not reliable for gene expression studies in equine adipose- and marrow-derived mesenchymal stem cells

Gene

Case N.

Replicates

BM-MSCs

AT-MSCs

   

C T mean

SD

C T mean

SD

GAPDH

3

3

19.11a

2.52

16.19b

0.49

ACTB

3

3

21.97a

2.21

18.05b

0.70

B2M

3

3

22.35a

3.14

17.66b

1.09

  1. Letters (a, b) represent significant differences in expression levels of GAPDH (P < 0.05), β-actin (P < 0.001) and B2M (P < 0.006) between BM- and AT-derived MSCs. BM = Bone marrow; AT = Adipose tissue; MSCs = Mesenchymal stem cells.