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Table 1 The RT-PCR and qRT-PCR primers used in this study

From: Expression patterns of TRα and CRABPII genes in Chinese cashmere goat skin during prenatal development

Primer Name

Sequence (5’-3’)

Fragment size (bp)

T.M. (°C)

Cloning primers

TRα-1 F

CCTGGATGGAATTGAAGTGA

799

62.0

TRα-1R

GACATGATCTCCATGCAGC

  

TRα-2 F

AGGCCTTCAGCGAGTTTAC

652

59.0

TRα-2R

CCTTCTCTCCAGGCTCCTC

  

CRABPII-1 F

CAGTGCTCCAGTGGAAAGA

563

56.5

CRABPII-1R

CCAGAAGTGATTGGGTGAG

  

Real-time PCR primers

TRα-3 F

TTACCTGGACAAAGACGAGC

113

57.4

TRα-3R

TCTGGATTGTGCGGCGAAAG

  

CRABPII-2 F

ACATCAAAACCTCCACCACC

111

56.5

CRABPII-2R

CCCATTTCACCAGGCTCTTA

  

ACTB-F

CCTGCGGCATTCACGAAACTAC

87

58.5

ACTB-R

ACAGCACCGTGTTGGCGTAGAG

  

GAPDH-F

GCA AGTTCCACGGCACAG

249

59.0

GAPDH-R

GGT TCACGCCCATCACAA

  

TOP2B-F

GTGTGGAGCCTGAGTGGTATA

137

59.0

TOP2B-R

AAGCATTCGCCTGACATTGTT