Table 1 Data of the factorial experiment with four and three levels of α -tocopherol and the maturation culture system, respectively
Maturation medium | α-tocopherol (μg/ml) | No. of oocytes (maturation/cleavage) | GVBD (% ± SEM) | MII (% ± SEM) | Cleavage (% ± SEM) |
|---|---|---|---|---|---|
MM | 0 | 122/88 | 18 (14.75 ± 3.40) | 80 (65.57 ± 5.84) | 32 (36.36 ± 7.37) |
5 | 115/120 | 15 (13.04 ± 2.49) | 81 (70.43 ± 4.28) | 43 (35.83 ± 4.77) | |
10 | 101/114 | 16 (15.84 ± 4.30) | 64 (63.37 ± 5.05) | 47 (41.23 ± 6.05) | |
15 | 118/106 | 27 (22.88 ± 3.43) | 67 (56.78 ± 2.47) | 32 (30.19 ± 1.57) | |
CG | 0 | 135/116 | 58 (42.96 ± 4.89) | 36 (26.67 ± 3.78) | 67 (57.76 ± 3.00) |
5 | 125/140 | 47 (37.60 ± 3.46) | 51 (40.80 ± 4.20) | 76 (54.29 ± 2.26) | |
10 | 135/119 | 54 (40.00 ± 3.07) | 52 (38.52 ± 1.21) | 55 (46.22 ± 3.16) | |
15 | 135/77 | 48 (35.55 ± 1.12) | 49 (36.30 ± 4.80) | 26 (33.77 ± 2.27) | |
CG + 12hMM | 0 | 131/130 | 11 (8.37 ± 3.45) | 112 (85.50 ± 3.37) | 79 (60.77 ± 4.08) |
5 | 131/91 | 6 (4.58 ± 1.88) | 115 (87.79 ± 4.17) | 47 (51.65 ± 1.53) | |
10 | 138/82 | 9 (6.51 ± 1.91) | 117 (84.78 ± 4.57) | 57 (69.51 ± 3.47) | |
15 | 126/119 | 11 (8.73 ± 2.73) | 105 (83.33 ± 1.30) | 57 (47.90 ± 1.03) |