In silico characterisation, homology modelling and structure-based functional annotation of blunt snout bream (Megalobrama amblycephala) Hsp70 and Hsc70 proteins

Table 4 Top five identified structural analogs in the Protein Data Bank (PDB) library

	PDB ID	Protein	Species	TM-score	RMSD^a	IDEN^a	Cov.
Ma-Hsp70	1yuwA	Hsc70	Bos taurus	0.986	0.95	0.896	0.996
	4j8fA	Hsc70	Rattus norvegicus	0.688	1.78	0.676	0.711
	3cqxB	Hsc70	Mus musculus	0.678	0.90	0.902	0.685
	3iucC	Hsp70	Homo sapiens	0.677	0.95	0.687	0.685
	3qmlA	Hsp70	Saccharomyces cerevisiae	0.669	1.17	0.656	0.682
Ma-Hsc70	1yuwA	Hsc70	Bos taurus	0.986	1.24	0.958	1.000
	4j8fA	Hsc70	Rattus norvegicus	0.688	1.81	0.654	0.711
	3cqxB	Hsc70	Mus musculus	0.678	0.90	0.960	0.685
	3iucC	Hsp70	Homo sapiens	0.677	0.95	0.698	0.685
	3qmlA	Hsp70	Saccharomyces cerevisiae	0.669	1.17	0.659	0.682

Analogs were inferred by COFACTOR analysis, based on the TM-score of the structural alignment between the query structure and known structures in the PDB. RMSD^a is the average root mean square deviation between residues that are structurally aligned by TM-align; IDEN^a is the percentage sequence identity in the structurally aligned region; Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein

ISSN: 2055-0391