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Table 2 Data of DMI, milk production, milk composition, and various serum variables related to carbohydrate metabolism as well as to proinflammatory cytokines and acute phase proteins at the diagnosis week, and prior to the diagnosis of subclinical mastitis (SCM) at -8 and -4 weeks prior to expected day of parturition

From: Innate immunity and carbohydrate metabolism alterations precede occurrence of subclinical mastitis in transition dairy cows

  -8 weeks before parturation 4 weeks before parturation SCM diagnosis weeka 4 weeks after parturition
Item CON SCM P-value CON SCM P-value CON SCM P-value CON P-value SCM P-value
DMI (kg/d) 16.27 ± 1.04 14.94 ± 1.04 0.42 15.93 ± 0.10 12.98 ± 0.75 0.05 20.34 ± 0.56 16.23 ± 0.44 <0.01 21.16 ± 0.68 22.46 ± 1.79 0.23
Milk production (kg/d)        43.01 ± 1.62 28.23 ± 3.95 <0.01 42.04 ± 2.21 35.36 ± 3.96 0.17
Milk composition (g/kg, unless otherwise stated)           
Fat        5.08 ± 0.45 3.32 ± 0.41 0.02    
Protein        3.00 ± 0.10 2.97 ± 0.09 0.86    
Fat : Protein ratio        1.69 ± 0.12 1.11 ± 0.12 <0.01    
Lactose        4.54 ± 0.05 4.53 ± 0.06 0.92    
SCC (103 cells/mL)        28.33 ± 5.63 1,867 ± 699 0.02    
Milk urea N (mg/dL)        15.39 ± 0.76 18.70 ± 1.11 0.03    
TS        12.21 ± 0.31 12.19 ± 0.26 0.95    
Serum parameters             
Lactate (μmol/L) 2,455 ± 349 3,478 ± 153 0.03 2,162 ± 184 3,467 ± 546 0.04 2,228 ± 320 2,215 ± 203 0.98 2,100 ± 129 2,999 ± 76 <0.01
NEFA (mmol/L) 141 ± 32.77 179 ± 42.91 0.51 194 ± 47.17 92.97 ± 10.42 0.09 756 ± 232 303 ± 55 0.11 500.43 ± 151.71 243.89 ± 39.28 0.16
BHBA (μmol/L) 352 ± 37.71 336 ± 42.62 0.78 312 ± 18.50 354 ± 3.32 0.08 827 ± 151 598 ± 176 0.35 896.73 ± 188.3 777.8 ± 91.43 0.58
IL-1 (pg/mL) 316.79 ± 6.04 308 ± 13.76 0.55 321 ± 1.59 306. ± 1.46 <0.01 277. ± 5.42 297 ± 4.50 0.02 270.57 ± 2.87 283.02 ± 7.22 0.14
IL-6 (pg/mL) 19.23 ± 5.67 13.23 ± 3.15 0.42 48.24 ± 17.51 14.75 ± 6.13 0.24 23.17 ± 5.18 23.43 ± 4.89 0.97 15.71 ± 3.27 21.73 ± 6.02 0.38
TNF (ng/mL) 0.34 ± 0.03 0.35 ± 0.02 0.96 0.27 ± 0.05 1.29 ± 0.05 <0.01 0.06 ± 0.03 0.23 ± 0.01 0.01 0.07 ± 0.04 0.28 ± 0.04 0.01
Haptoglobin (mg/mL) 0.19 ± 0.03 0.05 ± 0.01 <0.01 0.15 ± 0.01 0.07 ± 0.01 <0.01 0.12 ± 0.01 0.33 ± 0.02 <0.01 0.16 ± 0.01 0.10 ± 0.02 0.04
SAA (ug/mL) 8,448 ± 3,373 8,799 ± 1,464 0.96 3,461 ± 342 21,052 ± 12,162 0.39 10,401 ± 1722 33,705 ± 9,459 0.05 11,797 ± 1,853 31,419 ± 8,039 0.05
  1. aCows were diagnosed with subclinical mastitis (n = 6) ranging from week +2 to +3. CON = cows without subclinical mastitis (health control); SCM = cows with subclinical mastitis