Effect of misting and wallowing cooling systems on milk yield, blood and physiological variables during heat stress in lactating Murrah buffalo

Table 4 Effect of misting and wallowing on hematological parameters of heat stressed lactating Murrah buffaloes

Parameters		PCV (%)	Hb (g %)	TEC (10⁶/μl)	MCV (fL)	MCH (pg)	MCHC	TLC (10³/μl)	Neutro %	Lymph %	Mono %	Eosino %
Period (p value)		0.977	0.77	0.51	0.47	0.64	0.77	0.20	0.01*	0.002*	0.82	0.77
Treatment (p value)		0.01*	0.12	0.03*	0.06	0.08	0.06	<.0001*	<.0001*	<.0001*	<.0001*	0.63
Control		25.42^b	9.17	4.63 ^b	55.40	19.77	36.53	7.40^a	32.94 ^a	57.94 ^a	4.11^a	5.00
Misting		29.79 ^a	9.72	4.91 ^ab	51.47	19.76	32.81	7.03^b	27.83 ^b	62.83 ^b	4.17^a	5.17
Wallowing		30.45 ^a	10.33	5.30 ^a	60.64	17.48	33.94	6.99^b	26.61 ^b	63.50 ^b	5.17^b	4.72
SE for Treatment		1.17	0.39	0.17	2.61	0.81	1.11	0.03	0.43	0.38	0.14	0.33
Period*Treatment (p value)		0.96	0.99	0.92	0.60	0.64	0.86	0.10	0.01*	0.0004*	0.93	0.87
May	Control	26.33	9.40	4.82	57.88	19.46	36.32	7.25 ^ab	31.00 ^b	60.00 ^cd	4.00 ^b	5.00
	Misting	29.22	10.07	5.11	64.34	19.62	34.84	6.99^bc	26.67^cd	64.17^a	4.17 ^ab	5.00
	Wallowing	30.65	10.43	5.36	60.52	16.47	34.06	7.03 ^bc	26.33 ^cd	63.50 ^ab	5.17 ^a	5.00
June	Control	25.61	9.23	4.63	55.33	19.87	36.45	7.44 ^a	32.67 ^ab	58.17 ^de	4.00 ^b	5.17
	Misting	30.00	9.60	4.94	57.05	19.38	32.07	7.02 ^bc	29.50 ^bc	60.67 ^bcd	4.17 ^ab	5.67
	Wallowing	30.05	10.13	5.18	55.75	19.51	33.70	7.08 ^bc	27.50 ^cd	62.83 ^abc	5.17 ^a	4.50
July	Control	24.33	8.87	4.43	48.27	19.97	36.85	7.52^a	35.17 ^a	55.67 ^e	4.33 ^ab	4.83
	Misting	30.16	9.50	4.68	55.13	20.29	31.51	6.95 ^c	27.33 ^cd	63.67 ^ab	4.17 ^ab	4.83
	Wallowing	30.65	10.43	5.36	48.27	16.47	34.06	7.01 ^bc	26.00 ^d	64.17^a	5.17 ^a	4.67
SE for Period*Treatment		2.02	0.67	0.29	4.53	1.40	1.92	0.60	0.74	0.66	0.26	0.57

Effect of Period, treatment and interaction of Period and treatment are indicated with p values
Means within a column having different superscripts differ significantly
The standard error uses pooled estimate of error variance
PCV Packed Cell Volume, Hb Hemoglobin, TEC Total Erythrocyte Count, MCV Mean Corpuscular Volume, MCH Mean Corpuscular Hemoglobin, MCHC Mean Corpuscular Hemoglobin Concentration, TLC Total Leukocyte Count, Neutro Neutrophil, Mono Monocyte, Eosino Eosinophil, Baso Basophil