Effect of misting and wallowing cooling systems on milk yield, blood and physiological variables during heat stress in lactating Murrah buffalo

Table 5 Effect of misting and wallowing on serum metabolites, electrolytes, enzyme activity and redox status of heat stressed lactating Murrah buffaloes

Parameters		Urea	Creatinine	Glucose	Sodium	Potassium	Chloride	AST	AKP	SOD	ROS
Period (p value)		<.0001*	<.0001*	0.1132	0.15	0.0203*	0.26	<.0001*	<.0001*	0.0054*	<.0001*
Treatment (p value)		0.33	0.02*	0.2298	<.0001*	0.52	0.99	0.4618	0.95	0.48	0.65
Control		20.27	1.87^a	47.49	129.94 ^b	6.72	89.51	40.20	10.01	304.6	4.86
Misting		20.19	1.80 ^b	48.07	153.83 ^a	6.56	89.45	39.40	10.05	314.49	4.80
Wallowing		19.94	1.84 ^ab	47.78	152.83 ^a	6.89	89.47	39.88	10.03	315.16	4.86
SE for Treatment		0.16	0.02	0.23	0.70	0.20	0.27	0.45	0.10	6.79	0.05
Period*Treatment (p value)		0.94	0.64	0.30	0.04*	0.003*	0.99	0.71	0.68	0.35	0.64
May	Control	19.53 ^d	1.91 ^d	47.53	129.17 ^b	5.33 ^b	89.13	39.99 ^ab	10.23 ^a	308.80	4.56 ^b
	Misting	19.40^d	1.78 ^d	47.98	154.83 ^a	6.50 ^ab	89.10	38.03^b	10.38^a	305.60	4.60^b
	Wallowing	19.70^cd	1.84 ^cd	47.93	151.5 ^a	7.17 ^a	89.10	38.72 ^ab	10.43 ^a	305.80	4.71 ^b
June	Control	20.03 ^abcd	1.77 ^abcd	48.33	128.67 ^b	7.17 ^a	89.60	39.10 ^ab	10.62 ^a	298.90	4.69 ^b
	Misting	19.53 ^d	1.70 ^d	48.20	151.17 ^a	6.33 ^ab	89.53	38.53 ^ab	10.42 ^a	297.70	4.57 ^b
	Wallowing	19.90 ^bcd	1.75 ^bcd	47.80	154.50 ^a	6.50 ^ab	89.67	38.93 ^ab	10.54 ^a	296.83	4.63 ^b
July	Control	21.00 ^ab	1.93 ^a	46.60	132.0 ^b	7.67^a	89.80	41.50^ab	9.18 ^b	306.1	5.34 ^a
	Misting	20.90 ^abc	1.90 ^abc	48.02	155.50 ^a	6.83 ^ab	89.72	41.65 ^a	9.36^b	340.17	5.25^a
	Wallowing	21.20 ^a	1.93 ^a	47.60	152.50 ^a	7.00 ^a	89.63	42.00 ^a	9.11 ^b	342.83	5.25 ^a
SE for Period* Treatment		0.27	0.03	0.41	1.22	0.35	0.47	0.78	0.18	11.76	.08

Effect of Period, treatment and interaction of Period and treatment are indicated with p values
Means within a column having different superscripts differ significantly
Urea (mg/100 ml), Creatinine (mg/100 ml), Glucose (mg/100 ml), Sodium (meq/L), Potassium (meq/L), Chloride (mg/100 ml), AST Aspartate aminotransferase (IU/L), AKPAlkaline phosphatase (KA units), SOD Superoxide dismutase (U/ml), ROS Reactive oxygen species (mg H₂O₂ equivalents/ml)