Table 1 The information of primers for PCR and MassArray
Primer | Purpose | Primer sequence (5′ → 3′) | Amplicon size (bp) | Tm (°C) |
|---|---|---|---|---|
P1 | Amplify exon 1 | F: CTGCTTAGGGACTTGGTGCTGT | 690 | 58.0 |
R: AGCCCTTGGTGTAGAACTGTTTGA | ||||
P2 | c.72G>A genotyping | 1st: ACGTTGGATGTCCTTCCTCCTGCAGGCAC | 102 | 49.8 |
2nd: ACGTTGGATGATGAGGTCATAGGTGACCAG |