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Table 3 Antioxidant status, protein degradation and lipid oxidation in infraspinatus (IS) and gluteus medius (GM) muscles in goats during postmortem chill storage

From: Comparison of myofibrillar protein degradation, antioxidant profile, fatty acids, metmyoglobin reducing activity, physicochemical properties and sensory attributes of gluteus medius and infraspinatus muscles in goats

  

Storage time (days)

P value

Parameter

Muscle

0

1

4

7

Storage

Storage × muscle

α-tocopherol (mg/kg)

IS

2.19 ± 0.130a

2.01 ± 0.100b

1.43 ± 0.061c

1.16 ± 0.020c

<.0001

0.192

 

GM

2.14 ± 0.111a

1.93 ± 0.120b

1.40 ± 0.032c

1.23 ± 0.040c

0.0012

 
 

P value

0.217

0.140

0.223

0.123

  

γ-tocopherol (mg/kg)

IS

0.69 ± 0.051a

0.65 ± 0.052a

0.41 ± 0.010b

0.23 ± 0.020c

<.0001

0.237

 

GM

0.70 ± 0.022a

0.65 ± 0.021a

0.44 ± 0.040b

0.27 ± 0.010c

<.0001

 
 

P value

0.532

0.112

0.267

0.417

  

δ-tocopherol (mg/kg)

IS

0.08 ± 0.011

0.08 ± 0.012

0.07 ± 0.011

0.07 ± 0.011

0.109

0.280

 

GM

0.12 ± 0.031

0.10 ± 0.011

0.09 ± 0.021

0.09 ± 0.031

0.204

 
 

P value

0.211

0.234

0.091

0.094

  

Total carotenoid (mg/kg)

IS

0.27 ± 0.010a

0.23 ± 0.011a

0.18 ± 0.012b

0.11 ± 0.010c

<.0001

0.447

 

GM

0.25 ± 0.030a

0.22 ± 0.012a

0.15 ± 0.041b

0.10 ± 0.030c

0.004

 
 

P value

0.901

0.548

0.071

0.361

  

Catalase1

IS

1828.9 ± 27.23y

1820.0 ± 20.83y

1844.2 ± 21.91y

1816.3 ± 26.80y

0.190

0.135

 

GM

1666.2 ± 11.80x

1767.0 ± 10.20x

1515.2 ± 12.11x

1700.2 ± 17.10x

0.219

 
 

P value

0.012

0.009

0.034

0.022

  

SOD2

IS

3.14 ± 0.161y

3.07 ± 0.121y

3.31 ± 0.212y

3.17 ± 0.05y

0.742

0.221

 

GM

2.70 ± 0.132x

2.45 ± 0.143x

2.87 ± 0.111x

2.78 ± 0.05x

0.092

 
 

P value

0.030

0.002

0.043

0.012

  

GPX3

IS

66.75 ± 2.621

63.42 ± 3.500

65.03 ± 2.772

63.01 ± 0.940

0.806

0.156

 

GM

65.20 ± 1.600

59.89 ± 1.250

63.11 ± 2.112

62.22 ± 1.332

0.418

 
 

P value

0.190

0.090

0.301

0.128

  

TBARS (mg MDA/kg)

IS

0.12 ± 0.010c

0.15 ± 0.011c

0.19 ± 0.010b

0.28 ± 0.010a

<.0001

0.173

 

GM

0.11 ± 0.010c

0.13 ± 0.020c

0.18 ± 0.030b

0.26 ± 0.031a

<0.001

 
 

P value

0.130

0.273

0.112

0.367

  

Carbonyl (nmol/mg protein)

IS

1.08 ± 0.041a

1.43 ± 0.130a

2.47 ± 0.291b

3.63 ± 0.120cx

<.0001

0.451

GM

1.09 ± 0.010a

1.35 ± 0.120a

2.50 ± 0.122b

3.01 ± 0.221cy

<.0001

 
 

P value

0.231

0.154

0.347

0.035

  

Free thiol (nmol/mg protein)

IS

52.89 ± 1.671a

49.75 ± 1.302a

45.91 ± 1.380b

39.25 ± 1.711c

0.014

0.234

 

GM

55. 82 ± 1.220a

52.86 ± 1.112a

48.00 ± 1.091b

41.01 ± 1.000c

0.021

 
 

P value

0.213

0.437

0.815

0.200

  
  1. a, b c means having different superscripts along the same row are significantly different (P < 0.05). x, y means with different superscript along the same column are significantly different (P < 0.05). 1catalase activity is expressed as nmol.H2O2/min/mg protein. 2superoxide dismutase is expressed as the amount of enzyme needed to inhibit 50 % dismutation of the superoxide radical. 3glutathione peroxidase activity is expressed as nmoles NADPH oxidized/min/mg protein