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Table 4 The pH and concentration of NH3-N and VFA after in vitro ruminal fermentation

From: Evaluation of feed value of a by-product of pickled radish for ruminants: analyses of nutrient composition, storage stability, and in vitro ruminal fermentation

  Treatment1   P-value
Item CON T15 T30 T45 SEM Mean Linear Quadratic
pH
 3 h 6.58a 6.56ab 6.55b 6.55b 0.004 0.01 <0.01 0.08
 6 h 6.56a 6.54ab 6.54bc 6.52c 0.003 <0.01 <0.01 0.35
 12 h 6.51a 6.48b 6.45c 6.43d 0.003 <0.01 <0.01 0.63
 24 h 6.44 6.43 6.41 6.41 0.006 0.02 <0.01 0.81
 48 h 6.39a 6.38ab 6.36b 6.27b 0.005 0.02 <0.01 0.09
 72 h 6.40 6.40 6.40 6.40 0.005 0.75 0.89 0.75
NH3-N (mg/dL)
 3 h 10.97 10.02 9.41 8.80 0.662 0.20 0.04 0.81
 6 h 12.95a 12.76ab 12.24ab 11.80b 0.243 0.04 <0.01 0.63
 12 h 12.95 12.18 11.27 11.36 0.713 0.36 0.11 0.56
 24 h 15.22 15.32 16.15 15.49 0.504 0.59 0.49 0.48
 48 h 20.69b 22.54a 22.28a 23.77a 0.624 0.05 0.01 0.78
 72 h 27.12b 26.68b 29.53a 30.40a 0.352 <0.01 <0.01 0.10
Total VFA (mM)
 3 h 19.73 20.24 20.57 21.16 0.491 0.29 0.07 0.94
 6 h 23.32b 24.58ab 28.20a 27.85a 0.872 <0.01 <0.01 0.38
 12 h 31.54c 34.39bc 36.90ab 38.11a 0.668 <0.01 <0.01 0.25
 24 h 40.11 46.47 46.97 43.57 1.751 0.08 0.20 0.02
 48 h 51.08 53.99 53.61 51.93 2.255 0.77 0.83 0.34
 72 h 53.14b 55.06b 58.42a 60.17a 0.570 <0.01 <0.01 0.89
A:P ratio
 3 h 4.07a 3.97b 3.87c 3.83c 0.017 <0.01 <0.01 0.16
 6 h 4.07a 4.06a 3.95b 3.96b 0.014 <0.01 <0.01 0.46
 12 h 4.15a 4.13a 3.95b 3.94b 0.013 <0.01 <0.01 0.60
 24 h 3.77c 3.83bc 3.88ab 3.96a 0.019 <0.01 <0.01 0.58
 48 h 3.64c 3.72bc 3.79ab 3.85a 0.023 <0.01 <0.01 0.52
 72 h 3.60c 3.67b 3.73a 3.77a 0.010 <0.01 <0.01 0.15
  1. 1CON: Control diet consisting of 400 g/kg concentrate mix and 600 g/kg annual ryegrass straw; T15: 90 g/kg of annual ryegrass straw (15 %) was substituted with a by-product of pickled radish; T30: 180 g/kg of annual ryegrass straw (30 %) was substituted with a by-product of pickled radish; T45: 270 g/kg of annual ryegrass straw (45 %) was substituted with a by-product of pickled radish
  2. a-dMeans that do not have common superscript differ (P < 0.05)