Fig. 7

The elution profile of proteins of Vietnam cobra venom at gel filtration on a Superdex 75 column (left) and calibration curve for determining molecular weights of the enzyme (right). Standard proteins: 1. Bovine serum albumin (BSA, 67 kDa); 2. Ovalbumin (45 kDa); 3. Trypsin (24 kDa); 4. RNase A (13.7 kDa); 5. Insulin (5.8 kDa). Conditions of gel filtration: 10 mM phosphate buffer pH 7.4; flow rate 1 ml/min