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Table 3 Effect of cashew nut shell liquid and monensin supplementation under different dietary conditions on in vitro short chain fatty acid production

From: Potency of cashew nut shell liquid in rumen modulation under different dietary conditions and indication of its surfactant action against rumen bacteria

Parameters

Forage to concentrate ratio

P-value

9:1

7:3

5:5

3:7

1:9

Control

Treatment

Control

Treatment

Control

Treatment

Control

Treatment

Control

Treatment

Additive

Diet

A × D

 

mmol/dL

   

Cashew nut shell liquid

 Total SCFA

9.57c

9.81bc

9.64bc

10.67ab

10.26bc

11.36a

10.71ab

10.71ab

10.67ab

10.49abc

0.004

<0.001

0.019

 Acetate

6.44ab

6.41ab

6.40ab

6.70ab

6.72a

6.83a

6.82a

6.35bc

6.74a

6.12b

0.078

0.048

0.003

 Propionate

1.64e

2.11cde

1.71de

2.60b

1.90cde

3.32a

2.13bcd

3.30a

2.21bc

3.46a

<0.001

<0.001

<0.001

 n-Butyrate

1.13c

0.99de

1.20bc

1.07cd

1.30ab

0.94de

1.40a

0.84ef

1.36a

0.73f

<0.001

0.011

<0.001

Monensin

 Total SCFA

8.08b

7.96b

8.26b

8.29b

8.71ab

9.03ab

8.69ab

8.80ab

9.41a

8.73ab

0.650

<0.001

0.280

 Acetate

5.20ab

5.00ab

5.15ab

4.92ab

5.24ab

5.16ab

5.07ab

4.81ab

5.38a

4.69b

0.002

0.416

0.228

 Propionate

1.82cd

1.78d

1.92cd

2.16bcd

2.19bcd

2.65ab

2.33bc

2.91a

2.64ab

2.95a

<0.001

<0.001

0.093

 n-Butyrate

0.82d

0.90cd

0.95bcd

0.99abcd

1.03abc

1.04abc

1.09ab

0.92bcd

1.16a

0.93bcd

0.029

<0.001

0.001

 

molar %

   

Cashew nut shell liquid

 Acetate

67.28a

65.35b

66.39ab

62.73c

65.48b

60.13d

63.72c

59.30de

63.22c

58.37e

<0.001

<0.001

<0.001

 Propionate

17.17f

21.46d

17.74f

24.34c

18.47ef

29.16b

19.90de

30.67b

20.72d

32.99a

<0.001

<0.001

<0.001

 n-Butyrate

11.80b

10.03c

12.43ab

10.05c

12.63ab

8.31d

13.08a

7.87d

12.72ab

6.90e

<0.001

<0.001

<0.001

Monensin

 Acetate

64.21a

62.53a

62.33ab

59.33cd

60.12bc

57.19d

58.34cd

54.58e

57.23d

53.78e

<0.001

<0.001

0.280

 Propionate

22.51de

22.27e

23.21de

26.00cde

25.02cde

29.29bc

26.67cd

33.05ab

28.11c

33.79a

<0.001

<0.001

0.007

 n-Butyrate

10.19c

11.27abc

11.52abc

11.87abc

11.87abc

11.49abc

12.51a

10.47bc

12.37ab

10.57bc

0.034

0.119

0.001

  1. A × D indicates interaction between additive and diet effects
  2. a-fMeans within a row with different superscripts significantly differ (P < 0.05)