In vitro rumen fermentation kinetics, metabolite production, methane and substrate degradability of polyphenol rich plant leaves and their component complete feed blocks

Table 4 In vitro ruminal fermentation metabolites of different forages

Attributes	Roughage			SEM	Significance (P value)
Attributes	Vigna sinensis hay	Acacia nilotica leaves	Ziziphus nummularia leaves	SEM	Significance (P value)
Total N (mg/dL)	47.3^c	57.1^b	60.5^a	0.459	< 0.001
Ammonia N (mg/dL)	16.4^b	17.3^a	16.4^b	0.209	0.046
TCA precipitable N (mg/dL)	15.7^b	10.8^c	21.8^a	0.971	< 0.001
Acetic acid (mM/L)	26.3^b	31.3^a	11.1^c	0.759	< 0.001
Propionic acid (mM/L)	5.31^a	5.06^a	2.60^b	0.159	< 0.001
Isobutyric acid (mM/L)	0.88^a	0.89^a	0.26^b	0.021	< 0.001
Butyric acid (mM/L)	2.89^a	2.68^a	2.28^b	0.069	0.003
Isovaleric acid (mM/L)	0.75^a	0.89^a	0.24^b	0.039	< 0.001
Valeric acid (mM/L)	0.37	0.34	0.64	0.103	0.137
Total VFA (mM/L)	36.5^b	41.2^a	17.1^c	0.911	< 0.001
Branch-chain fatty acids (mM/L)	2.00^a	2.13^a	1.15^b	0.119	0.002
Acetate: propionate ratio	4.96^b	6.18^a	4.25^c	0.11	< 0.001
Non-glucogenic: glucogenic VFA ratio	4.66^b	5.40^a	4.47^b	0.11	0.002

ISSN: 2055-0391