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Table 4 In vitro ruminal fermentation metabolites of different forages

From: In vitro rumen fermentation kinetics, metabolite production, methane and substrate degradability of polyphenol rich plant leaves and their component complete feed blocks

Attributes Roughage SEM Significance (P value)
Vigna sinensis hay Acacia nilotica leaves Ziziphus nummularia leaves
Total N (mg/dL) 47.3c 57.1b 60.5a 0.459 < 0.001
Ammonia N (mg/dL) 16.4b 17.3a 16.4b 0.209 0.046
TCA precipitable N (mg/dL) 15.7b 10.8c 21.8a 0.971 < 0.001
Acetic acid (mM/L) 26.3b 31.3a 11.1c 0.759 < 0.001
Propionic acid (mM/L) 5.31a 5.06a 2.60b 0.159 < 0.001
Isobutyric acid (mM/L) 0.88a 0.89a 0.26b 0.021 < 0.001
Butyric acid (mM/L) 2.89a 2.68a 2.28b 0.069 0.003
Isovaleric acid (mM/L) 0.75a 0.89a 0.24b 0.039 < 0.001
Valeric acid (mM/L) 0.37 0.34 0.64 0.103 0.137
Total VFA (mM/L) 36.5b 41.2a 17.1c 0.911 < 0.001
Branch-chain fatty acids (mM/L) 2.00a 2.13a 1.15b 0.119 0.002
Acetate: propionate ratio 4.96b 6.18a 4.25c 0.11 < 0.001
Non-glucogenic: glucogenic VFA ratio 4.66b 5.40a 4.47b 0.11 0.002
  1. Means bearing different superscript (a, b, c) differ significantly
  2. TCA Trichloroaceticic acid, VFA Volatile fatty acids