In vitro rumen fermentation kinetics, metabolite production, methane and substrate degradability of polyphenol rich plant leaves and their component complete feed blocks

Table 5 In vitro ruminal fermentation metabolites of complete feed block (CFB)

Attributes	Complete feed block
Attributes	CFB1	CFB2	CFB3	SEM	Significance (P value)
Total N (mg/dL)	103^b	91.3^c	115^a	1.52	< 0.001
Ammonia N (mg/dL)	18.1^b	20.8^a	17.8^b	0.209	< 0.001
TCA precipitable N (mg/dL)	78.8^a	44.6^c	70.3^b	1.67	< 0.001
Acetic acid (mM/L)	29.8^a	33.6^a	17.9^b	1.73	< 0.001
Propionic acid (mM/L)	6.53^a	7.44^a	3.47^b	0.351	< 0.001
Isobutyric acid (mM/L)	0.95^a	1.00^a	0.37^b	0.029	< 0.001
Butyric acid (mM/L)	4.19^b	5.03^a	2.50^c	0.179	< 0.001
Isovaleric acid (mM/L)	0.89^b	1.09^a	0.29^c	0.029	< 0.001
Valeric acid (mM/L)	0.41^b	0.45^b	0.95^a	0.041	< 0.001
Total VFA (mM/L)	42.8^a	48.6^a	25.5^b	2.2	< 0.001
Branch-chain fatty acids (mM/L)	2.24^b	2.54^a	1.61^c	0.063	< 0.001
Acetate: propionate ratio	4.54^b	4.54^b	5.13^a	0.148	0.047
Nonglucogenic: glucogenic VFA ratio	4.59	4.64	4.82	0.108	0.38

Means bearing different superscript (^{a, b, c}) differ significantly
TCA Trichloroaceticic acid, VFA Volatile fatty acids
CFB1 Concentrate mixture + Vigna sinensis hay (70:30), CFB2 Concentrate mixture + Acacia nilotica leaves (70:30), CFB3 Concentrate mixture + Ziziphus nummularia leaves (70:30)

ISSN: 2055-0391