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Table 3 Effect of nZnO levels on the rumen fluid VFA (n = 4 observations/treatment)

From: In vitro evaluation of nano zinc oxide (nZnO) on mitigation of gaseous emissions

Effects

Alfalfa

Maize silage

Control

100 μg g−1

200 μg g− 1

500 μg g− 1

1000 μg g− 1

SEMa

P value

Feed

nZnO

nZnOa

Feed

Acetic Acid (mM)

108x

107x

122a

97.1b

98b

108ab

114a

14.15

0.832

0.005

0.172

Propionic Acid (mM)

33.05x

49.53y

50.88a

34.01c

36.94bc

41.11bc

43.50ab

7.68

<.0001

0.002

0.688

P/A ratio

0.306x

0.444y

0.423a

0.389a

0.369a

0.379a

0.380a

0.058

0.189

0.004

0.007

Total VFA (mM)

161x

173x

193a

149c

152bc

166bc

174ab

21.51

0.086

0.002

0.743

  1. aData are presented as least square means per treatment ± SEM
  2. Means followed by the letters (x/y/a/b/c/d) in each VFA type are not significantly different at P ≤ 0.05
  3. VFA Volatile Fatty Acid, P/A Propionic acid to Acetic acid ratio