Validation of a simple binary scoring system for assessment of welfare measures of 10-day-old commercial broilers and their correlation with environmental parameters
© Kumari et al.; licensee BioMed Central. 2015
Received: 13 November 2014
Accepted: 8 January 2015
Published: 7 March 2015
A simple binary scoring system (SBSS) was developed and used to assess the welfare measures of commercial broiler chickens in South Korea. We also correlated welfare measures with environmental parameters of broiler house. Our measures of welfare included lameness, hock burn (HB) and foot pad dermatitis (FPD), whilst environmental parameters included air temperature, relative humidity, air speed, light intensity, air quality (in particular carbon dioxide (CO2) and ammonia (NH3) concentrations) and airborne microbes.
The effect of environmental parameters on welfare measures was apparent even on 10-day-old broilers. A non-parametric correlation analysis revealed significant correlations between environmental parameters and welfare measures. The key environmental parameters were relative humidity and light intensity. The results indicate that there is a need for proper control of environmental conditions on poultry farms, which could reduce health problems and subsequently reduce disease and mortality.
In conclusion, the simplicity of SBSS makes it preferable over more complex scoring systems and allows a farmer to more easily assess the welfare measures on their own farm.
KeywordsAirborne microbes Air temperature Environmental parameters Welfare measures
Due to their short reproductive cycle and worldwide popularity as food animal production, poultry generally represent the most-highly selected type of livestock. The selection of broiler chickens has been primarily directed at economic specialty, which has reduced the costs of production . Throughout the world, the majority of broilers are reared in intensive production systems, where birds are confined for their lifetime under a high density stocking environment . They are reared from hatch to slaughter and weighed monthly. It may take less than 40 days to go from hatch to slaughter.
Because animal welfare assurance has become an important aspect of the marketing of poultry products to food retailers, the need has arisen for an appropriate method to measure welfare in commercial broiler flocks. Visual inspection of welfare measures offers the advantage of allowing a noninvasive evaluation of a large number of birds in a short period of time. A valid and feasible method for scoring broiler welfare on commercial farms is needed so that farmers can easily apply a consistent scoring method in a reasonable length of time. Presently, Welfare Quality assessment protocol of European Union has been used to assess welfare measures in breeder stocks and has been adapted for the evaluation of commercial broiler production . Welfare Quality assessment protocol defines 3 to 6 scoring categories on an ordinal scale of severity. The differences between categories are subtle enough to make scoring perhaps more difficult and slower than necessary in a commercial production environment. Therefore there is a need to develop a simple welfare assessment protocol, which will be simple enough for routine use under commercial conditions.
The internal environment of poultry buildings is a complex dynamic system influenced by many contributory factors. A number of these factors impact bird health, behavior and productivity. Environmental conditions are generally considered as the main factors affecting the welfare of broilers in farms. Environmental conditions that are thought crucial to broiler welfare include: temperature, humidity, light intensity, air speed and air quality [4-8]. Broilers release many harmful substances from their metabolism and various activities, such as carbon dioxide (CO2), ammonia (NH3) and airborne microbes [9-11]. To keep the broilers and workers healthy, the concentration of these pollutants in air should be monitored and reduced to safe limits.
A real-time simple binary scoring system (SBSS), which seeks to identify broilers as having no impairment (score 0) and having severe impairment (score 1). As discussed above, the present study aimed to test the effectiveness of SBSS by evaluating the welfare measures (lameness, hock burn (HB) and foot pad dermatitis (FPD) of commercial broilers in South Korea. We also assessed the impact of environmental parameters (air temperature, relative humidity, air speed, light intensity, air quality and airborne microbes) on these welfare measures of commercial broilers.
Site selection and experimental design
Details of management and husbandry data from broiler house
House type and wall type
H-Beam and sandwich panel
Solid with bedding material was rice husks
Date of cleaning of floor
5/ 15/ 2013 (cleaning per month after sell)
Temperature (air and body)
33°C (air) and 39°C (body)
Total number of birds on site (at the time of visit)
Total number of birds originally placed in house
23000 (total 90000 = 23000 + 17000 + 17000 + 17000 + 16000)
Total number of birds in house (at the time of the visit)
Age at day of inspection
MD: 1 day, IB: 7 day, IBD: 14 day
Parent flock age(s)
Average bird weight at time of visit (taken from records of weights taken by the producer)
344 g (10 day after birth)
Feed quality and time interval
Feed bought from Jeil Feed company and feeding was operated automatically
Feed withdrawal rate
Number of stock workers
Vaccination for disease, Time of vaccination.
MD: 1 day, IB: 7 day, IBD: 14 day
In which month calls rate is high and why (throughout the year)
November to February because of cold
In which month mortality rate is high and why (throughout the year)
November to February because of cold
Sampling points and measurement of environmental parameters
All the welfare measures and environmental parameters were measured at nine points inside a chicken house at 15 cm above the floor, which corresponded to the nose height of the chickens and data was collected in triplicate from each point. The arithmetic means and SD of the each variable were calculated before further statistical analysis. Air temperature and relative humidity were measured with a hygrothermograph (SK-110TRH, SATO, Tokyo, Japan). Air speed was measured with an anemometer (model 6112, KANOMAX, Osaka, Japan). Light intensity (lux) values were recorded using a light meter (ANR-F9 LUX METER, Tokyo Photo-electric Co. Ltd, Japan) held at arm’s length and at bird height. The greenhouse gases concentrations in particular CO2 and NH3 were measured using a GASTECH device (Pump kit No. 101). A GASTECH device was used despite having several limitations because it is simple to handle and a typical farmer can easily identify the various compounds with the help of this instrument.
Airborne bacterial analysis
Airborne bacterial counts were measured using a settle-plate method. This is a direct method for assessing the likely number of microorganisms depositing onto the product or surface in a given time. It is based on the fact that in the absence of any kind of influence, airborne microorganisms, typically attached to larger particles, will deposit onto open culture plates. Tryptic soy agar (Merck, Darmstadt, Germany) was used for enumeration of total airborne bacteria, and Chromocult Coliformen agar (Merck, Darmstadt, Germany) was used for Total Coliforms and Escherichia coli. After sampling, the plates were incubated at 37°C for 48 h, and the colonies were counted and calculated as colony-forming units. Samples were collected from nine points in triplicate.
Assessment of welfare measures
Scoring of lameness, foot pad dermatitis and hock burn
Absence of lameness
Presence of lameness
Foot pad dermatitis
No discoloration or lesions
Hock with lesion
The details of management, husbandry and environmental parameters data were evaluated under the guidelines of Ross Broiler Management Manual . The statistical evaluation was carried out with an SPSS software package (SPSS Inc., Chicago, IL). Pairwise correlations (Kendall’s τ-b correlation coefficients) were calculated among environmental parameters and welfare measures.
Results and discussion
Environmental parameters in broiler house
Environmental parameters measured inside broiler house
Air temperature (°C)
Air speed (m/s)
Relative humidity (%)
Light intensity (lux)
Jones et al.  emphasized the importance of temperature and relative humidity to the health and mortality of broiler chickens produced in a northern European climate by analyzing the temperature and humidity profiles throughout the growth cycle. Jones et al.  found that high temperature and relative humidity adversely affect the lameness, FPD and mortality. Air speed has also been shown to significantly affect broiler performance . Deep et al.  showed that light intensity did not affect broiler production and mortality, but did affect carcass characteristics.
The mean concentrations of CO2, and NH3 were 1755.5 and 4.0 ppm, respectively, in the house within the farm across nine different points (Table 3). The concentrations of these gases were within the recommended range suggested for broiler houses . CO2, originating from animal respiration as well as from manure breakdown, is an important gas in confined animal buildings. Concentrations and emissions of CO2 have sometimes been used to estimate poultry house ventilation rates [14,15]. High concentrations of NH3 inside animal houses represent potential health hazards to humans and animals . The mean concentration of TAB, TC and TE in the broiler houses were 277.1, 53.6 and 7.2 (cfu/m3), respectively (Table 3). It has been shown that dead and partially decomposed air microbes may cause inflammation in the respiratory organs, and antigens and allergens may activate the immune system, leading to allergic reactions [17,18].
Welfare measures of broiler house
Welfare measures evaluated from broiler house
Lameness score 0 (%)
Lameness score 1 (%)
Hock score 0 (%)
Hock score 1 (%)
Footpad score 0 (%)
Footpad score 1 (%)
Correlation between environmental parameters and welfare measures
Kendall’s τ-b correlation coefficients for environmental parameters with welfare measures
Lameness score 1
Hock score 0
Hock score 1
Footpad score 0
Footpad score 1
The aim of our study was to apply the SBSS to assess the welfare measures of broilers and to evaluate the effect of environmental parameters on these welfare measures. Our results indicate that SBSS is a quick and simple scoring method for evaluating broiler welfare measures. This method could help in taking quick decisions about farm management maintenance issues, which could subsequently reduce disease and mortality and enhance broiler production. We also concluded that the control of the environment for broiler chickens is a key factor in improving their welfare, particularly through the control of relative humidity and light intensity.
This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries (IPET) from the Ministry of Agriculture, Food, and Rural Affairs (MAFRA) through project no. 312036-03-2-HD030 and in part by Korea Institute of Energy Technology Evaluation and Planning (KETEP) from the Ministry of Trade, Industry and Energy (MOTIE) through project no. 2012–3020090040.
- Emmans G, Kyriazakis I. Consequences of genetic change in farm animals on food intake and feeding behaviour. Proc Nutr Soc. 2001;60:115–25.View ArticlePubMedGoogle Scholar
- Otte J, Roland-Holst D, Pfeiffer D, Soares-Magalhaes R, Rushton J, Graham J, et al. Industrial livestock production and global health risks. In: Food and Agriculture Organization of the United Nations, Pro-Poor Livestock Policy Initiative Research Report. 2007.Google Scholar
- Quality W. Welfare Quality® assessment protocol for poultry (broilers, laying hens). Lelystad, the Netherlands: Welfare Quality® Consortium; 2009.Google Scholar
- Jones T, Donnelly C, Dawkins MS. Environmental and management factors affecting the welfare of chickens on commercial farms in the United Kingdom and Denmark stocked at five densities. Poult Sci. 2005;84:1155–65.View ArticlePubMedGoogle Scholar
- Dawkins MS, Donnelly CA, Jones TA. Chicken welfare is influenced more by housing conditions than by stocking density. Nature. 2004;427:342–4.View ArticlePubMedGoogle Scholar
- Meluzzi A, Fabbri C, Folegatti E, Sirri F. Survey of chicken rearing conditions in Italy: effects of litter quality and stocking density on productivity, foot dermatitis and carcase injuries. Br Poult Sci. 2008;49:257–64.View ArticlePubMedGoogle Scholar
- May J, Lott B. The effect of environmental temperature on growth and feed conversion of broilers to 21 days of age. Poult Sci. 2000;79:669–71.View ArticlePubMedGoogle Scholar
- Yahav S. Relative humidity at moderate ambient temperatures: its effect on male broiler chickens and turkeys. Br Poult Sci. 2000;41:94–100.View ArticlePubMedGoogle Scholar
- Osorio JA, Ferreira Tinoco I, Ciro HJ. Ammonia: A review of concentration and emmision model in levestock structures. Dyna. 2009;76:89–99.Google Scholar
- Cambra-López M, Aarnink AJ, Zhao Y, Calvet S, Torres AG. Airborne particulate matter from livestock production systems: A review of an air pollution problem. Environ Pollut. 2010;158:1–17.View ArticlePubMedGoogle Scholar
- Tegethoff V, Hartung J. A field study on stocking density and air quality in broiler production and recommendations to avoid heat stress in summer. Dtsch Tierarztl Wochenschr. 1996;103:87–91.PubMedGoogle Scholar
- Avigen. ROSS Broiler Management Manual. Newbridge, Scottland: Aviagen Ltd; 2002.Google Scholar
- Deep A, Schwean-Lardner K, Crowe T, Fancher B, Classen H. Effect of light intensity on broiler production, processing characteristics, and welfare. Poult Sci. 2010;89:2326–33.View ArticlePubMedGoogle Scholar
- Groot Koerkamp P, Metz J, Uenk G, Phillips V, Holden M, Sneath R, et al. Concentrations and emissions of ammonia in livestock buildings in Northern Europe. J Agr Eng Res. 1998;70:79–95.View ArticleGoogle Scholar
- Liang Y, Xin H, Li H, Wheeler EF, Zajaczkowski JL, Topper PA, et al. Ammonia emissions from US laying hen houses in Iowa and Pennsylvania. T ASAE. 2005;48:1927.View ArticleGoogle Scholar
- Portejoie S, Martinez J, Landmann G. L’ammoniac d’origine agricole: impacts sur la santé humaine et animale et sur le milieu naturel. INRA Prod Anim. 2002;15:151–60.Google Scholar
- Douwes J, Thorne P, Pearce N, Heederik D. Bioaerosol health effects and exposure assessment: progress and prospects. Ann Occup Hyg. 2003;47:187–200.View ArticlePubMedGoogle Scholar
- Schenker MB, Christiani D, Cormier Y, Dimich-Ward H, Doekes G, Dosman J, et al. American Thoracic Society: respiratory health hazards in agriculture. Am J Respir Crit Care Med. 1998;158:S1–76.View ArticleGoogle Scholar
- Berg CC. Foot-pad dermatitis in broilers and turkeys. In: Doctoral Thesis. Diss. (sammanfattning/summary) Skara: Sveriges lantbruksuniv, Acta Universitatis agriculturae Sueciae. Veterinaria; 1998. p. 1401–6257. 1998:36.Google Scholar
- Blatchford R, Klasing K, Shivaprasad H, Wakenell P, Archer G, Mench J. The effect of light intensity on the behavior, eye and leg health, and immune function of broiler chickens. Poult Sci. 2009;88:20–8.View ArticlePubMedGoogle Scholar
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