Thymic Hassall’s corpuscles in Nandanam chicken - light and electronmicroscopic perspective (Gallus domesticus)
© Kannan et al. 2015
Received: 26 May 2015
Accepted: 22 August 2015
Published: 1 October 2015
The present study was aimed to study the light and electron microscopic studies of thymic Hassall’s corpuscles was done in various age groups of Nandanam Chicken ranging from day-old to forty weeks. Hassall’s corpuscles are special, unique structures present in thymic medulla and also in the cortex of all the age groups of Nandanam chicken (from hatch to forty weeks) in the present study. Size of the Hassall’s corpuscles in the medulla is larger than the ones present in the cortical region of thymus. The Hassall’s corpuscles are made up of structureless eosinophilic mass surrounded by concentrically arranged reticuloepithelial cells. Under electron microscope, the Hassall’s corpuscles were composed of reticuloepithelial cells interconnected by many desmosomes. The epithelial cells had abundance of cytoplasmic fibrils and desmosomes with few mitochondria and ribosomes. The nucleus was oval or round which was slightly indented. The centre of the Hassall’s corpuscles was appeared either solid or cystic. The cystic corpuscles had cell debris within the cyst lumen.
KeywordsLight and electronmicroscopy Thymus Hassall’s corpuscles Nandanam Chicken
All living beings manage not only to survive but indeed thrive in potentially hostile milieu, without seeming effort. This freedom from disease is depends on the existence of a complex and highly sophisticated defense system, called lymphatic system [1, 2].
Thymus is a central lymphoid organ in which bone marrow-derived T-cell precursors undergo differentiation, maturation and eventually leading to migration of positively selected thymocytes to the peripheral lymphoid organs [3, 4]. Thymus differs from other lymphoid organs as it undergoes involution as age advances .
In chicken, thymic parenchyma is madeup of an outer darker cortex and inner paler medulla . Hassall’s corpuscles are characteristic structure of thymic medulla, first demonstrated by Arthur Hill Hassall who described it as an acidophilic, squamous spherical structures in the thymic medulla, unique to this organ [5, 7–9].
There are several studies highlighted the existence of differences between thymic Hassall’s corpuscles of mammals and birds [10–12]. Nandanam chicken is a dual purpose, colored variety with good disease resistance and most popular among poultry farmers due to its adaptability to backyard farming. This strain was developed in Institute of Poultry Production and Management, Tamil Nadu Veterinary and Animal Sciences University, Chennai. Hence, the present study has been designed with aim of exploring the light and electronmicroscopic structural details of thymic Hassall’s corpuscles in Nandanam chicken.
Over 36 specimens of thymic tissue were collected from six different age groups such as day-old, four, eight, twelve, twenty and forty weeks. Six birds were used in each age group. The thymus was removed immediately after high cervical dislocation and fixed for light and electron microscopy .
For light microscopic studies, tissue pieces were fixed in 10 % neutral buffered formalin and processed for paraffin (Cat. No. 8002-74-2 Sigma-Aldrich, India) embedding technique . Tissue sections were cut at 5 micron thickness and used for routine Haematoxylin-eosin staining method .
For electronmicroscopic study, small pieces of thymic tissue (1–2 mm thickness) were collected and prefixed at 3 % glutaraldehyde (Cat. No. G-5882, Sigma-Aldrich, India) and stored at 4 °C. Subsequent processing, sectioning of tissue and staining was done as per . The ultra thin sections were examined under Phillips (Teknai-10) computer augmented transmission electron microscope operated at 60-kilowatt ampere (KVA).
Procurement of birds for this study was approved by Institutional Animal Ethical Committee, Madras Veterianry College, Chennai-07.
Results and discussion
The number of corpuscles was found to be more as age advanced in the present study  which indicated the involutory changes in aged birds . The corpuscles were normally associated with blood sinusoids.
The association of dieing cells and macrophages with Hassall’s corpuscles in the present study proved beyond doubt that Hassall’s corpuscles were the repository for a great number of old cells [26, 27]. This finding is contrary to the findings in guinea pig where they opined that the Hassall’s corpuscles are the privileged areas for maturation of the medullary lymphocytes .
In the present study, two types of vesicles or cysts were observed in association with the Hassall’s corpuscles in all the age groups. Many epithelial cells in the medulla formed the unicellular or intracellular and multicellular or intercellular cysts. The cytoplasm of the intracellular cysts contained mitochondria, endoplasmic reticulum, Golgi apparatus, ribosomes, some small dense granules. The nucleus was pale, oval and had one or two nucleoli. These cystic cells were found attached to the neighbouring epithelial cells by desmosomes.
The intercellular cysts were found to be formed by two or three cells which varied from very pale to very dark in their appearance. The cytoplasm contained a few mitochondria and ribosomes and very little rough endoplasmic reticulum. The nucleus was found to be oval or spherical. The microvilli was seen projected into the lumen of the cyst. Some of the cysts appeared to be empty while some of them contained mucous droplets in this type of cyst also .
In the present study, the cells of the intercellular cysts contained dense secretory granules which are similar to the findings in chicken . This is good evidence to support the idea that the thymus is an endocrine gland, and secretes a hormone that induces lymphoid cells to acquire immunological competence . Whereas, it was opined that that cystic epithelial cells showed cytoplasmic features suggestive of a secretory function [31, 32].
Also some evidence to suggest that cystic epithelial cells in the mouse thymus secrete a sulphated mucoid lymphopoietic hormone . It was reported that a mucoid substance is regularly secreted by the epithelial cells forming the Hassall’s corpuscles in the human thymus . Whereas, it was suggested that “clear vesicles” present in reticulum cells of the rat thymus are the morphological equivalent of the production of a humoral thymus factor . However, [36, 37] considered that the granular inclusions in cystic epithelial cells may be related to a lymphocyte stimulating hormone. It was suggested that dense granules present in epithelial cells in the guinea pig thymus were kerato-hyaline granules linked to keratinisation but he did not exclude the possibility of the presence of another form of endocrine secretion, especially a colloid or steroid secretion .
Hassall’s corpuscles were unique structure of thymus, present in both cortex and medulla. Number of Hassall’s corpuscles increased as age advances. The corpuscles were madeup of homogenous, eosinophilic mass surrounded by concentrically arranged reticuloepithelial cells. In the present study, two types of cysts, intracellular and intercellular cysts were observed in association with the Hassall’s corpuscles in all the age groups. Intracellular cyst was either free or associated with the Hassall’s corpuscles were common in all the age groups. Intercellular cysts were noticed in advanced age groups of chicken.
The authors are thankful to the Dean, Madras Veterinary College for providing facilities to carry out this work.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Cortan RS, Kumar V, Robbin SL. Robbin’s Pathogenic Basis of Disease. 4th ed. Philadelphia: W.B. Saunders; 1998. p. 163–4.Google Scholar
- Khan MZI, Masum A, Khan MZI, Aziz ARB, Nasrin M, Siddiqi MNH, et al. Histomorphology of the Lymphoid Tissues of Broiler Chickens in Kelantan, Malaysia. Sains Malaysiana. 2014;43(8):1175–9.Google Scholar
- Savino W, Dardenne M. Neuroendocrine control of thymus physiology. Endocr Rev. 2000;21:412–43.PubMedGoogle Scholar
- Ciriaco E, Pinera PP, Diaz-Esnal B, Laura R. Age related changes in the avian primary lymphoid organs (Thymus and Bursa of fabricius). Micros Res Tech. 2003;62:482–87.View ArticleGoogle Scholar
- Hassan AU, Rasool Z. The Hassal of Thymus: Hassals Corpuscle Histological and Histopathological Perspective. Sch J App Med Sci. 2014;2(1B):147–8.Google Scholar
- Treesh SA, Buker AO, Khair NS. Histological, histochemical and immunohistochemical studies on thymus of chicken. Int J Histol Cytol. 2014;1(11):103–11.Google Scholar
- Ortiz-Hidalgo C. Early clinical pathologists 5: The man behind Hassall’s corpuscles. J Clin Pathol. 1992;45:99–101.PubMed CentralView ArticlePubMedGoogle Scholar
- Schuurman HJ, Kuper CF, Kendall MD. Thymic microenvironment at the light microscopic level. Micros Res Tech. 1997;38:216–26.View ArticleGoogle Scholar
- Varga I, Pospisilova V, Jablonska V, Sisovsky V, Galfiova P, Polak S, et al. Thymic Hassall’s bodies of children with congenital defects. Bratisl Lek Listy. 2010;111(10):552–7.PubMedGoogle Scholar
- Watanabe N, Wang YH, Lee HK, Ito T, Wang YH, Cao W, et al. Hassall’s corpuscles instruct dendritic cells to induce CD4+CD25+ regulatory T cells in human thymus. Nature. 2005;436:1181–5.View ArticlePubMedGoogle Scholar
- Miclăuș V, Petrescu-Mag IV, Rus V, Cadar D, Oana L, Ober C. Frequency and polymorphism of Hassall’s corpuscles in normal thymus of nutria (Myocastor coypus). AACL Bioflux. 2009;2(2):121–31.Google Scholar
- Rus V, Gal A, Ruxanda F, Boșca B, Nadăș G, Rațiu C, et al. Morphostructural Characterization of Hassall’s Corpuscles in Lamb. Bulletin UASVM Vet Med. 2014;71(2):1–6.Google Scholar
- Gilmore RSC, Bridges JB. Histological and ultrastructural studies on the myoid cells of the thymus of the domestic fowl, Gallus domesticus. J Anat. 1974;118:409–16.PubMed CentralPubMedGoogle Scholar
- Bancroft JD, Layton C, Suvarna SK. Bancroft’s Theory and Practice of Histological Techniques. 7th ed. Elsevier: Churchill Livingstone; 2013.Google Scholar
- Kannan TA, Ramesh G, Ushakumari S, Dhinakarraj G, Vairamuthu S. Light microscopic studies on Spleen of Chicken (Gallus domesticus). Haryana Vet. 2012;51(12):114–5.Google Scholar
- Kannan TA, Geetha R, Ushakumari S, Dhinakarraj G, Vairamuthu S. Electron Microscopic Studies of Spleen in Chicken (Gallus domesticus) (Gallus domesticus). Int J of Adv Vet Sci Tech. 2015;4(1):160–5.Google Scholar
- Trautmann A, Fiebiger J. Fundamentals of the histology of domestic animals. New York: Comstock Pub. Assoc; 1957. p. 142–43.Google Scholar
- Leena C, Prasad RV, Kakade K, Jamuna KV. Histology and age related involutary changes of the thymus of Giriraja birds (Gallus domesticus). J Vet Anim Sci. 2008;39:40–3.Google Scholar
- Haseeb A, Shah MG, Gandahi JA, Lochi GM, Khan MS, Faisal M, et al. Histo-morphological Study on Thymus of Aseel chicken. J Agric Food Tech. 2014;4(2):1–5.Google Scholar
- Kendall MD, Singh J. The presence of erythroid cells in the thymus gland of man. J Anat. 1980;130:183–89.PubMed CentralPubMedGoogle Scholar
- Tarek K, Mohamed M, Omar B, Hassina B. Morpho-Histological study of the thymus of broiler chickens during Post-Hashing Age. Int J Poult Sci. 2012;11(1):78–80.View ArticleGoogle Scholar
- Bridges JB, Gilmore PR, Morris GP. The ultrastructure of the thymus of the cockrel. J Anat. 1970;107:388–89.Google Scholar
- Hashimoto Y, Sugimura M. Histological and quantitative studies on the postnatal growth of the thymus and the bursa of Fabricius of white Pekin ducks. Jap J Vet Res. 1976;24:65–76.Google Scholar
- Robert MV, Bensch KG, Levin GD. The normal human thymic vasculature: An ultrastructural study. Anat Rec. 1978;183:485–98.Google Scholar
- Frazier JA. Ultrastructure of the chick thymus. Zellgasch. 1973;136:191–205.Google Scholar
- Blau JN. Hassall’s corpuscles- a sight of thymocyte death. Br J Exp Path. 1973;54:634–7.Google Scholar
- Olsson L, Classon MH. Studies on the regulation of lymphocyte production in the murine thymus and some effects of crude thymus extract. Cell Tis Res. 1975;8:491.Google Scholar
- Senelar R, Escola MJ, Serron B, Seree A. Relationship between Hassall’s corpuscles and thymocytes in guinea pig foetus. Biomedicine. 1976;24:112–22.PubMedGoogle Scholar
- Chan AS. Ultrastructure of epithelial thymic cysts of the chicks. Poult Sci. 1986;65:177–82.View ArticlePubMedGoogle Scholar
- Goldstein AL, Asanuma Y, White A. The thymus as an endocrine gland: properties of thymosin, a new thymus hormone. Recent Progr Hormone Res. 1970;26:505–38.PubMedGoogle Scholar
- Hoshino T. Electron microscopic studies of the epithelial reticular cells of the mouse thymus. Z Zellforsch. 1963;59:513–29.View ArticlePubMedGoogle Scholar
- van Haelst U. Light and electronmicroscopic study of the normal and pathological thymus of the rat. I the normal thymus. Z Zellforsch Mikrosk Anat. 1967;77:534–53.View ArticlePubMedGoogle Scholar
- Clark Jr SL. The thymus in mice of strain 129/J studied with the electron microscope. Americian J Anat. 1963;112:1–33.View ArticleGoogle Scholar
- Henry K. Mucin secretion and striated muscles in the human thymus. Lancet. 1966;I:183–85.View ArticleGoogle Scholar
- Pfoch M. Vergleichende elektronenmikroskopische Untersuchung an entodermalen Thymus-Retikulumzellen neugeborener und alter Wistar-Ratten. Z Zellforsch. 1971;114:271–80.View ArticlePubMedGoogle Scholar
- Cesarini JP, Benkoel L, Bonneau H. Ultrastructure compare du thymus chez le hamsterjeune et adulte. C R Soe Biol (Paris). 1968;162:1975–79.Google Scholar
- Chapman WL, Allen JR. The fine structure of the thymus of the fetal and neonatal monkey (Macaca mulatta). Z Zellforsch. 1971;114:220–33.View ArticlePubMedGoogle Scholar
- Izard J. Ultrastructure of the thymic reticulum in G. Pig Anat Rec. 1966;155:117–32.View ArticlePubMedGoogle Scholar