- Open Access
Lesser known indigenous vegetables as potential natural egg colourant in laying chickens
© Abiodun et al.; licensee BioMed Central Ltd. 2014
- Received: 6 August 2014
- Accepted: 22 September 2014
- Published: 24 September 2014
A six-week study involving two hundred and fifty (250) Harco Black layer birds at point of lay was conducted to investigate the effects of potential natural colorant on performance and Egg quality traits. The birds were assigned to five (5) dietary treatments, each containing supplements either of control, Baobab Leaf (BL), Waterleaf (WL), Red Pepper (RP), Canthaxanthin (CTX) at 40 g/kg feed and 50 mg/kg feed of natural and commercial colorants, respectively.
Performance records shows that there was no significant (p > 0.05) difference in feed intake across the supplements of Red pepper, Water leaf, Canthaxanthin and control diet, however, birds fed Baobab leaf treatment had a significantly lower (p < 0.05) feed intake value (94.07 g) when compared with other treatments. Body weight gain and Hen Day Production were not significant influenced (p > 0.05) by the dietary treatments, although laying hens fed Baobab leaf supplement had lowest mean HDP of 48.80%, while birds fed Red pepper and Water leaf supplement had an average value of 52.79%. There was no significant effect (p > 0.05) of colorants on egg external traits, compared with the control; birds fed Canthaxanthin treatment had higher mean egg weight (51.79 g), egg length (4.55 g), egg breadth (3.29 g); Red pepper treatment had highest mean shell thickness (0.29 g), however these differences were not significant (p > 0.05). Yolk height, Albumen height, Yolk index, and Haugh unit were not significantly affected (p > 0.05) across treatments. Yolk width was lowest (p < 0.05) in Baobab leaf treatment (2.54 cm); Red pepper, Water leaf and Canthaxanthin (2.89 cm, 2.62 cm and 2.89 cm respectively) were not significantly (p > 0.05) different from the control (2.73 cm). Yolk colour score was significantly highest (p < 0.05) in Red pepper treatment (7.50); Water leaf, Baobab leaf and Canthaxanthin ranged between 2.25- 3.31 on the DSM yolk colour fan, Control treatment had the lowest yolk colour score (p < 0.05) of 1.31.
The study showed Red pepper as a worthy alternative to commercial yolk colorant. Water leaf and baobab are not good substitutes for canthaxanthin as a yolk colourant.
- Egg yolk colorant
- Red pepper
- Baobab leaf
Eggs are used in various food industries in the manufacture of confectionery cosmetics, production of vaccine as reported by . Egg yolk colour is a major concern to consumers as it affects their purchasing behavior . The colour of the egg yolk is considered to be one of the important factors for egg consumption. Consumers select eggs based on the egg yolk color and other egg qualities. However, laying birds cannot produce coloring pigments that is known to enhance yolk coloration to meet the demand of consumer for the desired colored egg yolk and egg quality and thus, depends directly on dietary supply for egg yolk coloration . Indeed, the perception of the intensity of the yolk colour depends directly on the quantity of feed consumed, the transfer efficacy and chemical composition of the carotenoid source . The efficiency of pigment source depends on the digestibility, transfer, metabolism, and deposition of carotenoids in target tissue and upon their colour hue . The most effective carotenoids in poultry diets are the synthetic forms which have been manufactured to ensure high transfer efficiency and colouring capacity (apoester: 50-60%; canthaxanthin 30-50%) . The main vegetable sources of carotenoids are corn, corn gluten, lucerne, lucerne concentrates and flower (marigolds, tagetes) and plant (paprika) extracts. Synthetic oxy-carotenoids which corresponded to natural carotenoids (canthaxanthin, citranaxathin) have been chosen in the laying hen rations for their colouring effectiveness, (which is two to three times more efficient as a yolk colorant than carotenoids of vegetable origin), and for their high stability (due to encapsulation against oxidation and degradation) . These products are, however, banned in organic production, which rely mainly on yellower plant sources, explaining the paler yolk colouration found in this system of poultry production. Canthaxanthin has also been reported as a potential skin and eye irritant, and it is an expensive source of yolk colorant . In practice, satisfactory colour of table eggs can be obtained with small amounts of yellow xanthophylls (15–25 mg/kg) combined with 1–2 mg/kg red carotenoids.
The attractive red color of peppers, yellow color of ripe pawpaw and the green pigment of vegetables (Talinium triangulare, Telfairia occidentalis, Adansonia digitata, etc.) is due to their various carotenoid pigment. These carotenoids include; capsanthin, capsorubin and cryptocapsin . Maize (lutein), Red pepper (capsanthin and capsorubin), Fluted pumpkin, Pawpaw (cryptoxanthin), Sweet potato leaf and Marigold (lutein) contain colorants that can be used as feed supplements in diet of laying birds and are known to improve egg quality and yolk pigmentation.
Vegetable sources provide mainly yellow carotenoids. Paprika oleoresin is prepared from the dried fruit of red pepper by a similar process to tagetes involving dehydration, solvent extraction, saponification and stabilization are useful carotenoids for egg yolk coloration. Its effectiveness ratios relative to canthaxanthin varies from 3 to 5 for yolk deposition yield and 1 to 4 for pigmentation ability. Extract from algae containing Asthaxanthin (3,3′-dihydroxycanthaxanthin) has been used in combination with yellow xanthophylls for egg yolk coloration, but the rate of deposition of carotenoids in the egg yolk is lower than that of canthaxanthin .
This study focused on the use of lesser known indigenous vegetables as natural pigmenting plants; Red pepper (Capsicum annuum), Water leaf (Talinum triangulare) and Baobab leaf (Adansonia digitata), with the aim of improving egg quality traits through egg yolk coloration.
Experimental animals and management
The experiment was carried out at the Teaching and Research farm, Faculty of Agriculture, University of Ilorin. Two hundred and Fifty (250) twenty-week old Black Harco layers were used for the experiment that lasted six weeks. The laying hens were housed in a 2-tiers battery cage system. Diet was formulated to meet NRC  requirements for energy and protein of laying birds. Each natural plant colorants (Red Pepper, Baobab Leaf and Waterleaf) and a commercial yolk colorant (Canthaxanthin) were incorporated into the diet at separate rates of 40 g/kg and 50 mg/kg of feed, respectively. Other routine management practices such as vaccination, medication and proper hygiene recommended by animal science regulations in Nigeria were complied with.
Composition of experimental diet
Brewer’s dried grain
Fish meal (72%)
F1 = left over feed at the end of the day (g)
W1 = weight change at the end of the week
W0 = weight at the beginning of the week
At the end of each week, fifty eggs per treatment were selected for analysis. Parameters measured include; Egg Weight (EW), Egg Length (EL), Egg Breadth (EB), Yolk Height (YH), Yolk Width (YW), Albumen Height (AH), Shell Thickness (ST), Yolk Colour (YC), Yolk Index (YI)and Haugh Unit (HU).
EW was collected by cleaning the eggs to remove impurities and weighing them using a sensitive electronic scale (Metler). ST was determined using a pair of micrometer screw gauge calibrated in millimetres. The accuracy of ST was ensured by measuring shell sample as one egg at the broad end, middle portion, and the narrow end all referred to as the thin, medium and thick, respectively. The average of these three parts measured was taken as the ST. YI was determined by relating the ratio of YH in millimetres to the YW measured in millimetres. The YH and YW were measured using a Spherometer and Venier calliper, respectively. YW was also taken as the maximum cross sectional diameter of the yolk which is the width at maximum point, usually across the centre of the yolk. The YC of collected eggs were determined using DSM yolk colour fan.
H.U = Haugh unit
H = Observed albumen height.
W = Observed weight of egg in grams.
Data obtained from the experiment were subjected to analysis of variance for completely randomized experimental design using the SPSS package, according to . Significant means were separated using the Duncan’s Multiple Range Test .
Effects of potential natural colorant on bird’s performance
The relative effects of the potential natural colorant on Bird’s performance
950 ± 0.06ab
110 ± 0.03b
470 ± 0.26a
630 ± 0.15ab
750 ± 0.45ab
101.33 ± 3.32b
103.16 ± 3.13b
94.07 ± 1.37a
103.56 ± 1.39b
104.36 ± 2.24b
52.97 ± 17.99
52.97 ± 19.09
48.80 ± 16.22
58.34 ± 18.91
58.60 ± 20.08
HDP increased comparatively over the period of experiment. Laying hens fed with the control diet had the highest mean value (58.60%) while the diet supplemented with BL had the lowest value (48.8%). RP supplemented diet did not influence (P > 0.05) the performance parameters measured viz: Body weight gain, feed intake, Hen-day production. The observation on HDP disagreed with the report of  that CTX increased egg production. CTX has been reported to have no effects on HDP .
Effects of potential natural colorant on egg external quality traits
48.27 ± 1.20
47.56 ± 6.68
46.87 ± 5.20
51.79 ± 2.10
49.84 ± 2.34
4.35 ± 0.06
4.24 ± 0.60
4.20 ± 0.46
4.55 ± 0.16
4.37 ± 0.13
3.23 ± 0.09
3.08 ± 0.51
3.02 ± 0.39
3.29 ± 0.06
3.16 ± 0.30
1.35 ± 0.48
1.38 ± 0.63
1.39 ± 0.03
1.38 ± 0.06
1.38 ± 0.05
0.29 ± 0.18
0.27 ± 0.18
0.27 ± 0.44
0.28 ± 0.02
0.27 ± 0.03
The various dietary treatments had no significant effect (P > 0.05) on egg internal qualities such as: AH, YH, YI and HU. This is also consistent with earlier reports .
Effects of natural colorant on internal egg quality traits
Yolk height (mm)
14.83 ± 0.05
13.30 ± 2.07
13.75 ± 1.58
14.53 ± 0.52
14.62 ± 0.42
5.15 ± 0.84
4.81 ± 0.76
5.19 ± 0.86
5.04 ± 0.11
5.37 ± 0.14
Yolk width (cm)
2.89 ± 0.06
2.62 ± 0.37
2.54 ± 0.25
2.89 ± 0.12
2.73 ± 0.64
Albumen height (mm)
5.49 ± 0.21
5.06 ± 1.04
5.41 ± 1.19
5.78 ± 0.43
5.54 ± 0.30
77.41 ± 1.68
76.08 ± 2.22
78.21 ± 4.03
77.54 ± 2.58
76.93 ± 2.25
7.50 ± 1.47c
3.31 ± 0.83b
3.06 ± 0.13b
2.25 ± 0.20ab
1.31 ± 0.13a
This study shows that lesser known indigenous vegetables are potential natural egg yolk colorants. RP supplement had superior performance in this regard over canthaxanthin (imported commercial egg yolk colourant). The period of importation and post-importation storage may have reduced the potency of CTX. The use of natural colorant such as lesser known indigenous vegetables is of utmost importance to improve yolk pigmentation and performance of birds with no adverse effect as compared to artificial colourants which could be of environmental and public health concern. Powdered RP supplementation could be used as an alternative for synthetic commercial yolk colorant which is quite expensive and potentially unhealthy. The use of yellow maize based diet with powdered WL and BL supplement can also help improve yolk color and performance.
- Oluyemi JA, Roberts FA: Poultry production in warm wet climates. 2007, PP 118-PP 120. London: Macmillan Press LtdGoogle Scholar
- Fletcher DL: Broiler breast meat colour variation, pH, and texture. Poult Sci. 1999, 78: 1323-1327. 10.1093/ps/78.9.1323View ArticlePubMedGoogle Scholar
- Soto-Salanova MF: Natural pigments: practical experiences. Recent Advances in Animal Nutrition. Edited by: Garnworty PC, Wiseman J. 2003, Nottingham, UK: Nothingham University PressGoogle Scholar
- Nys Y: Dietary carotenoids and egg yolk coloration. Archiv für Geflugelkunde. 2000, 64: 45-54.Google Scholar
- Hamilton PB: The use of high-performance liquid chromatography for studying pigmentation. Bri Poult Sci. 1992, 71: 718-724. 10.3382/ps.0710718.View ArticleGoogle Scholar
- Cho JH, Zhang ZF, Kim IH: Effects of Canthaxanthin on egg production, egg quality and Egg yolk color in laying hens. J Agric Sci. 2014, 5 (1): 269-274.Google Scholar
- Deepa N, Kaur C, George B, Singh B, Kapoor HC: Antioxidant constituents in some sweet pepper (Capsicum anuum L.) genotypes during maturity. LWT. 2007, 40: 121-128. 10.1016/j.lwt.2005.09.016.View ArticleGoogle Scholar
- EFSA: Scientific opinion on the safety and efficacy of canthaxanthin as a feed additive for poultry and for ornamental birds and ornamental fish. EFSA J. 2014, 12 (1): 3527-[24 pp.]. doi:10.2903/j.efsa.2014.3527. http://www.efsa.europa.eu/en/efsajournal/pub/3527.htm, Google Scholar
- Marusich WL, Bauernfeind JC: Oxycarotenoids in poultry feeds. Carotenoids as Colorants and Vitamin A Precursors. Edited by: Bauerfeind JC. 1981, 320-462. New York: Academic PressGoogle Scholar
- NRC: 9th Revised edition. Nutrient Requirements of Poultry. 1994, Washington, DC: National Academic PressGoogle Scholar
- Steel RGD, Torrie JH: Principle and Procedure of Statistics. 1982, London: McGraw- Hill, 2Google Scholar
- Duncan DB: Multiple range and F- test. Biometrics. 1955, 11: 1-42. 10.2307/3001478.View ArticleGoogle Scholar
- Butswat IS, Nelson FN, Oyawole EO, Akande FO: Utilization of baobab leaf meal for egg yolk pigmentation in layers. Ind J Animal Sci. 1997, 67 (1): 82-83.Google Scholar
- Kanda L, Koh-en Y, Tsutomu K, Keiko S: Enhancement of yolk colour in raw and boiled egg yolk with lutein from marigold flower meal and marigold flower extract. J Poultry Sci. 2001, 48: 25-32.Google Scholar
- Hasin BM, Feudaus AJM, Islam MA, Uddin MJ, Islam MS: Marigold and orange skin as egg yolk color promoting agents. Int J Poult Sci. 2006, 5: 979-987.View ArticleGoogle Scholar
- Khan M, Khatun M, Kibria A: Study the quality of eggs of different genotypes of chicken under scavenging system at Bangladesh. Pak J Biol Sci. 2004, 7 (12): 2163-2166.View ArticleGoogle Scholar
- Colin GS, George B, Ensminger ME: Poultry Science. 2004, New Jersey: Pearson Prentice Hall, Upper Saddle River, 4Google Scholar
- Niu Z, Fu J, Gao Y, Liu F: Influence of paprika extract supplement on egg quality of laying hens fed wheat-based diet. Int J Poultry Sci. 2008, 7: 887-889. 10.3923/ijps.2008.887.889.http://dx.doi.org/10.3923/ijps.2008.887.889, View ArticleGoogle Scholar
- Gouveia L, Veloso V, Reis A, Fernandes H, Novais J, Empis J: Chlorella vularis used to colouregg yolk. J Sci Food Agr. 1996, 70: 167-172. 10.1002/(SICI)1097-0010(199602)70:2<167::AID-JSFA472>3.0.CO;2-2.http://dx.doi.org/10.1002/(SICI)1097-0010(199602)70:2<167::AID-JSFA472>3.0.CO;2,View ArticleGoogle Scholar
- Selma UA, Miah G, Tareq KMA, Maki T, Tsujii H: Effects of dietary Rhodobacter capsulatus on egg-yolk cholesterol and laying hen performance. Poultry Sci. 2002, 86: 714-719.View ArticleGoogle Scholar
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