- Research
- Open Access
Estimation of genetic relationships between growth curve parameters in Guilan sheep
- Navid Ghavi Hossein-Zadeh^{1}Email author
https://doi.org/10.1186/s40781-015-0052-6
© Ghavi Hossein-Zadeh; licensee BioMed Central. 2015
- Received: 23 December 2014
- Accepted: 28 April 2015
- Published: 14 May 2015
Abstract
The objective of this study was to estimate variance components and genetic parameters for growth curve parameters in Guilan sheep. Studied traits were parameters of Brody growth model which included A (asymptotic mature weight), B (initial animal weight) and K (maturation rate). The data set and pedigree information used in this study were obtained from the Agricultural Organization of Guilan province (Rasht, Iran) and comprised 8647 growth curve records of lambs from birth to 240 days of age during 1994 to 2014. Marginal posterior distributions of parameters and variance components were estimated using TM program. The Gibbs sampler was run 300000 rounds and the first 60000 rounds were discarded as a burn-in period. Posterior mean estimates of direct heritabilities for A, B and K were 0.39, 0.23 and 0.039, respectively. Estimates of direct genetic correlation between growth curve parameters were 0.57, 0.03 and −0.01 between A-B, A-K and B-K, respectively. Estimates of direct genetic trends for A, B and K were positive and their corresponding values were 0.014 ± 0.003 (P < 0.001), 0.0012 ± 0.0009 (P > 0.05) and 0.000002 ± 0.0001 (P > 0.05), respectively. Residual correlations between growth curve parameters varied form −0.52 (between A-K) to 0.48 (between A-B). Also, phenotypic correlations between growth curve parameters varied form −0.49 (between A-K) to 0.47 (between A-B). The results of this study indicated that improvement of growth curve parameters of Guilan sheep seems feasible in selection programs. It is worthwhile to develop a selection strategy to obtain an appropriate shape of growth curve through changing genetically the parameters of growth model.
Keywords
- Bayesian methodology
- Fat-tailed sheep
- Genetic parameters
- Genetic trend
- Growth curve
Background
Growth is an economically important trait of farm animals which can be interpreted mathematically. It is a time (age) dependent change expressed in the weight or size of organ, composition of tissue/organ, size or number of cells and in live weight [1]. Growth rate is related to rate of maturing and mature weight and these traits have been suggested to have association with lifetime productivity parameters in animals [2]. Slow growth rate causes low market weight and has been identified as one of the limiting factors affecting the profitability of any production system [3].
Growth models have been used extensively in different species to describe the development of body weight, allowing information from multiple measurements to be combined into a few (usually 3 or 4) variables [4]. The fit of a growth function, and hence the variables estimated, will depend on the number and timing of available body weight observations [4]. Among a number of non-linear mathematical models, Brody function [5] has been found to be adequate for comparing individual differences in rate of maturing and mature weight. In addition, this model has advantages of mathematical simplicity and biological interpretability. Recently, other researchers have applied this model for fitting growth curve in sheep [3,6,7].
Accurate estimates of variance components are important in animal breeding because genetic parameters are dependent on (co) variance components [8]. Therefore, improved methodologies for estimation of these components are constantly evolving. The Gibbs Sampling algorithm [9] enables estimation of random samples of parameter estimates based on a given data set, by generating posterior distributions of parameter values that are proportional to the product of the parameter probabilities and the observation probabilities [10].
The ability to modify the shape of the growth curve may be an attractive aspect for livestock breeders (e.g., to increase early growth but restrict mature size, and hence maintenance requirements). To determine the genetic flexibility of the shape of growth curves, genetic parameters must be calculated for the underlying curve variables [4]. Estimates of genetic parameters and genetic trends for body weight traits in Guilan sheep were reported in a previous study [11], but estimates of variance components and heritabilities for growth curve parameters in sheep are very scarce in the literature. The objective of this study was to estimate variance components and genetic parameters for growth curve parameters in Guilan sheep using Gibbs sampling algorithm by Bayesian methodology. The results of the current study would indicate the possibility of implementing genetic selection for growth curve parameters in Guilan sheep. Also, current estimates of genetic parameters could be used for designing future selection schemes for Guilan breed, with the genetic improvement of growth rate being one of the main parts of selection goals.
Methods
The Guilan sheep is a fat-tailed breed of domestic sheep in Iran, numbering some 400,000 animals in the north of the country, and distributed in the northern and western parts of Guilan Province. Mean adult live weight in this breed is 35 kg (77 lbs) for rams and 31 kg (67 lbs) for ewes. This breed is valued mainly due to its ability to live in mountainous areas with rain-fed foothills and foothill steppes. Young ewes were randomly exposed to the rams for the first time at approximately 1.5 years of age. Ewes were kept in the flock up to 7 years of age. Ewes are supplemented, depending upon the ewes’ requirements, for a few days after lambing. During the breeding season, single-sire pens were used allocating 20–25 ewes per ram. Lambs remained with their dam until weaning. Lambs were ear-tagged and weighted immediately after lambing. During the suckling period, lambs suckled their mothers while being allowed dry alfalfa after 3 weeks of age. Lambs are weaned at approximately 90 days of age. Animals are kept on natural pasture during spring, summer and autumn seasons. Since environmental conditions are not suitable during the winter, therefore the animals are kept indoors during the winter months. The flock was mainly kept on range and fed cereal pasture, but supplemental feed, including alfalfa and wheat straw, are provided especially around mating season [12].
Where: y represents body weight at age t (days); A represents asymptotic weight, which is interpreted as mature weight; and B is an integration constant related to initial animal weight. The value of B is defined by the initial values for y and t; and K is the maturation rate, which is interpreted as weight change in relation to mature weight to indicate how fast the animal approaches adult weight. The data were screened several times and atypical growth curves resulting in out of range parameters were deleted. Lambs with incomplete records of parentage or with registration numbers lower than the numbers of their parents were left out. Averages of A, B and K parameters were 29.37 ± 9.67 kg, 0.89 ± 0.05 kg/day and 0.0069 ± 0.0035 kg gain per kg live weight per day, respectively. The whole number of animals, with sires, dams, founders and non-founders included in the pedigree data set were 21736, 304, 7154, 10275 and 11461, respectively.
Where, y is a N × 1 vector of records for each growth curve parameter, b denotes the fixed effects in the model with association matrix X, a is the vector of direct genetic effects with the incidence matrix Z and e denotes the vector of residual effects. From a Bayesian perspective, it was assumed that prior distribution for additive direct effects had a multivariate normal distribution with mean 0 and variance A σ _{ a } ^{2} , where A is the additive numerator relationship matrix and σ _{ a } ^{2} is the additive direct variance. Residual effects were assumed to be multivariate normally distributed with mean 0 and variance I _{ n } σ _{ e } ^{2} , where I _{ n } is identity matrix with order equal to the number of individual records, respectively and σ _{ e } ^{2} is the residual variance. Bivariate or pair-wise analyses were carried out for every pair of traits. The models applied in pair-wise analyses were those fitted for each of the traits in the univariate analyses.
The prior distribution for the genetic and residual (co)variance matrices was assumed to be an inverted Wishart distribution. Marginal posterior distributions of parameters and variance components were estimated, using TM program [14]. The Gibbs sampler was run 300000 rounds, and the first 60000 rounds were discarded as a burn-in period [14]. A thinning interval of 100 rounds was used to retain sampled values that reduced lag correlation among thinned samples. The Bayesian Output Analysis (BOA version 1.1.5; [15]) package was used to calculate the mean, SD, and 95% high posterior density (HPD) interval for all parameters from the individual marginal posteriors, under the free software R (http://www.r-project.org/). The HPD region or confidence interval provides the interval that includes 95% of samples and is a measure of reliability. Also, the HPD can be applied to non-symmetric distributions [16]. Genetic trends were obtained by regressing yearly mean estimates of breeding values on birth year. Phenotypic trends of growth curve parameters were estimated using the linear regression of average phenotypic values on birth year.
Results and discussion
The posterior mean, median and mode of direct genetic, residual and phenotypic variances and direct heritabilities for growth curve parameters of Guilan sheep [standard deviations (PSD) of marginal posterior distribution of mean estimates are in brackets]
Parameter | Item | Mean | Median | Mode | HPD |
---|---|---|---|---|---|
A | \( {\sigma}_a^2 \) | 32.38 (3.50) | 32.40 | 29.85 | 25.77-39.51 |
\( {\sigma}_e^2 \) | 50.74 (3.34) | 50.87 | 48.91 | 44.23-57.18 | |
\( {\sigma}_p^2 \) | 83.13 (1.32) | 83.09 | 83.03 | 80.50-85.66 | |
\( {h}_a^2 \) | 0.39 (0.04) | 0.39 | 0.37 | 0.31-0.47 | |
B | \( {\sigma}_a^2 \) | 5.86 (1.70) | 5.86 | 4.46 | 2.62-8.91 |
\( {\sigma}_e^2 \) | 19.37 (1.66) | 19.35 | 19.13 | 16.31-22.54 | |
\( {\sigma}_p^2 \) | 25.23 (0.42) | 25.24 | 25.09 | 24.47-26.05 | |
\( {h}_a^2 \) | 0.23 (0.07) | 0.23 | 0.24 | 0.10-0.35 | |
K | \( {\sigma}_a^2 \) | 0.39 (0.19) | 0.36 | 0.19 | 0.11-0.78 |
\( {\sigma}_e^2 \) | 9.64 (0.25) | 9.66 | 9.73 | 9.15-10.09 | |
\( {\sigma}_p^2 \) | 10.03 (0.16) | 10.03 | 9.99 | 9.74-10.35 | |
\( {h}_a^2 \) | 0.039 (0.019) | 0.035 | 0.019 | 0.011-0.078 |
The posterior mean, median and mode of genetic, residual and phenotypic correlations between growth curve parameters of Guilan sheep [standard deviations (PSD) of marginal posterior distribution of mean estimates are in brackets]
Correlation | Item | Traits | ||
---|---|---|---|---|
A-B | A-K | B-K | ||
\( {r}_{a_1{a}_2} \) | Mean | 0.57 (0.22) | 0.03 (0.34) | −0.01 (0.32) |
Median | 0.57 | 0.05 | 0.00 | |
Mode | 0.55 | 0.25 | 0.04 | |
HPD | 0.20-0.98 | −0.61-0.61 | −0.67-0.58 | |
\( {r}_{e_1{e}_2} \) | Mean | 0.48 (0.03) | −0.52 (0.02) | −0.32 (0.03) |
Median | 0.48 | −0.52 | −0.32 | |
Mode | 0.48 | −0.52 | −0.32 | |
HPD | 0.42-0.53 | −0.55- -0.48 | −0.37- -0.27 | |
\( {r}_{p_1{p}_2} \) | Mean | 0.47 (0.01) | −0.49 (0.01) | −0.28 (0.01) |
Median | 0.47 | −0.49 | −0.28 | |
Mode | 0.47 | −0.49 | −0.28 | |
HPD | 0.46-0.49 | −0.50- -0.47 | −0.30- -0.26 |
Estimates of direct genetic trends for A, B and K parameters were positive with values of 0.014 ± 0.003 (P < 0.001), 0.0012 ± 0.0009 (P > 0.05) and 0.000002 ± 0.0001 (P > 0.05), respectively. Estimates of phenotypic trends for A, B and K parameters were significantly positive (P < 0.001), the corresponding values being 0.12 ± 0.02, 0.22 ± 0.01 and 0.07 ± 0.01, respectively. There were no estimates of genetic trends for sheep growth curve parameters in literature. Accurate prediction of the breeding values of animals is one of the best tools available to maximize response to a selection plan [17]. The success of a breeding program can be assessed by testing the actual change in breeding value expressed as a proportion of expected theoretical change in the mean of breeding value for the trait under selection [24]. The positive and significant direct genetic trend for A parameter in this study indicates that the genetic merit for this parameter was increased over the years in Guilan sheep.
Conclusions
Current genetic estimates for growth curve parameters in Guilan sheep could be applied in designing selection program in this breed. Positive and significant direct genetic trend for A growth curve parameter of Guilan sheep was obtained in the current study. A and B parameters had the highest estimates of direct heritability and a high genetic correlation, they may be improved by genetic selection and could be included in genetic improvement programs for this breed. Also, it is important to develop an optimal selection strategy to achieve a desired shape of growth curve through changing genetically the parameters of model.
Declarations
Acknowledgement
The author would like to acknowledge the University of Guilan for financial support of this research (Project No. 989). Also, author wishes to thank the Agricultural organization of Guilan province for providing the data used in this study.
Authors’ Affiliations
References
- Eisen EJ. Result of growth curve analysis in mice and rats. J Anim Sci. 1976;42:1008–23.PubMedGoogle Scholar
- Pala A, Savas T, Ugur F, Das G. Growth curves of Turkish Saanen goats’ kids grouped for weight and body mass index. Arch Tierz. 2005;48:185–93.Google Scholar
- Abegaz S, Van Wyk JB, Olivier JJ. Estimation of genetic and phenotypic parameters of growth curve and their relationship with early growth and productivity in Horro sheep. Arch Tierz. 2010;53(1):85–94.Google Scholar
- Lambe NR, Navajas EA, Simm G, Bünger L. A genetic investigation of various growth models to describe growth of lambs of two contrasting breeds. J Anim Sci. 2006;84:2642–54.PubMedView ArticleGoogle Scholar
- Brody S. Bioenergetics and growth. NY, USA: Reinhold Publishing Corp; 1945.Google Scholar
- Gbangboche AB, Gleke-Kalai R, Albuquerque LG, Leroy P. Comparison of non-linear growth models to describe the growth curve in West African Dwarf sheep. Animal. 2008;2:1003–12.PubMedView ArticleGoogle Scholar
- Karakus K, Eyduran E, Kum D, Ozdemir T, Cengiz F. Determination of the best growth curve and measurement interval in Norduz male lambs. J Anim Vet Adv. 2008;7:1464–6.Google Scholar
- Schaeffer LR. Sire and cow evaluation under multiple trait models. J Dairy Sci. 1984;67:1567–73.View ArticleGoogle Scholar
- Magnabosco CU, Lôbo RB, Famula TR. Bayesian influence for genetic parameter estimation on growth traits for Nellore cattle in brazil, using the gibbs sample. J Anim Breed Genet. 2000;117:169–88.View ArticleGoogle Scholar
- Ghavi Hossein-Zadeh N, Ardalan M. Estimation of genetic parameters for body weight traits and litter size of Moghani sheep, using a Bayesian approach via Gibbs sampling. J Agr Sci. 2010;148:363–70.View ArticleGoogle Scholar
- Eteqadi B, Ghavi Hossein-Zadeh N, Shadparvar AA. Genetic analysis of body weight in native sheep of Guilan province. Anim Prod Res. 2015;3(4):63–73.Google Scholar
- Eteqadi B, Ghavi Hossein-Zadeh N, Shadparvar AA. Population structure and inbreeding effects on body weight traits of Guilan sheep in Iran. Small Rumin Res. 2014;119:45–51.View ArticleGoogle Scholar
- SAS. SAS User’s guide v. 9.1. Cary, NC: Statistics. SAS Institute, Inc; 2002.Google Scholar
- Legarra A, Varona L, Lopez de Maturana E. TM user’s guide; 2011. Retrieved May 03, 2015, from http://snp.toulouse.inra.fr/~alegarra.
- Smith BJ. Bayesian Output Analysis Program (BOA), Version 1.1.5. The University of Iowa. 2005. http://www.public-health.uiowa.edu/boa. Accessed Nov. 04, 2014.
- Hyndman RJ. Computing and graphing highest density regions. Am Stat. 1996;50:120–6.Google Scholar
- Ghavi Hossein-Zadeh N. Genetic parameters and trends for calving interval in the first three lactations of Iranian Holsteins. Trop Anim Health Prod. 2011;43:1111–5.PubMedView ArticleGoogle Scholar
- van der Westhuizen RR, Schoeman SJ, Jordaan GF, Van Wyk JB. Genetic parameters for reproductive traits in a beef cattle herd estimated using multitrait analysis. South Afr J Anim Sci. 2001;31:41–8.Google Scholar
- Bathaei SS, Leroy PL. Genetic and phenotypic aspects of the growth curve characteristics in Mehraban Iranian fat-tailed sheep. Small Rumin Res. 1998;29:261–9.View ArticleGoogle Scholar
- Stobart RH. Genetic and phenotypic analysis of liveweight maturing patterns and their relationship to ewe productivity in Colombia, Rambouillet and Targhee sheep. Dissert Abstr Int B-Sci Eng. 1983;44(6):1656.Google Scholar
- Stobart RH, Bassett JW, Cartwright TC, Blackwell RL. An analysis of body weights and maturing patterns in western range ewes. J Anim Sci. 1986;63:729–40.PubMedGoogle Scholar
- Mavrogenis AP, Constantinou A. Relationships between pre-weaning growth, post-weaning growth and mature body size in Chios sheep. Anim Prod. 1990;50(2):271–5.View ArticleGoogle Scholar
- Näsholm A. Mature weight of ewe as a trait in sheep breeding. Proceedings of the 4th World Congress on Genetics applied to Livestock Production, Edinburgh, 23–27 July, 1990. XV. Beef cattle, sheep and pig genetics and breeding, fiber, fur and meat quality. Edinburgh, UK: International Committee for World Congresses on Genetics Applied to Livestock Production; 1990, pp. 88–91.Google Scholar
- Jurado JJ, Alonso A, Alenda R. Selection response for growth in Spanish Merino flock. J Anim Sci. 1994;72:1433–40.PubMedGoogle Scholar
Copyright
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.