Confirmation of genotypic effects for the bovine APM1 gene on marbling in Hanwoo cattle
© Kwon et al. 2016
Received: 25 September 2015
Accepted: 23 March 2016
Published: 6 April 2016
Our previous study had identified the SNP (g.81966377T > C) and indel (g.81966364D > I) located in the promoter of APM1 to have a significant effect on marbling in Hanwoo. APM1 encodes an adipocytokine called adiponectin, which plays a significant role in lipogenesis. The aim of this study was to verify and validate the effect of the SNP and indel on marbling and other carcass traits in a large, representative, countrywide population of Hanwoo cattle. The carcass traits measured were marbling (MAR), backfat thickness (BFT), loin eye area (LEA), and carcass weight (CAW).
Primers were designed to amplify 346 bp of the genomic segment that contained the targeted SNP (g.81966377) and the indel (g.81966364). After data curation, the genotypes of 8,378 individuals identified using direct sequencing analysis estimated frequencies for C (0.686) and T (0.314) respectively showing genotype frequencies for CC (0.470), CT (0.430) and TT (0.098). The genotypes were significantly associated with MAR, BFT and LEA. The indel had significant effect on marbling (P < .0001) with strong additive genetic effects. The allele frequencies was estimated at (DEL, 0.864) and insertion (INS, 0.136) presenting genotypes of D/D (75.63 %), D/I (21.44 %), and I/I (2.92 %). Significant departure from Hardy-Weinberg equilibrium was not detected for both the SNP and the indel.
The SNP genotypes showed significant association with MAR, BFT and LEA with strong additive genetic effects, while the indel was significantly associated with MAR. The results confirmed that the variants can be used as a genetic marker for improving marbling in Hanwoo.
APM1 (Adipose most abundant gene transcript 1) gene codes an adipocytokine called adiponectin, and is secreted from the white adipose tissues [1, 2]. It is involved in lipogenesis, glucose production, insulin sensitivity, inflammatory response and blood circulation [3–5]. The gene is located on the bovine chromosome 1, is 13,256 bp in length and is composed of four exons, and the APM1 protein is 247 amino acids long . APM1 was identified to be located nearby the QTL affecting marbling, loin eye area and backfat thickness on BTA1 in Angus . Marker assisted selection has evolved as an important tool in selective breeding for developing high quality beef cattle.
Hanwoo which was a draught animal was selectively bred as beef cattle, with a strong emphasis on meat yield and quality traits . Beef quality in Korea is graded based on intramuscular fat or marbling (MAR), meat color (MCO), fat color (FCO), maturity (MAT), texture (TXT), carcass weight (CAW), back fat thickness (BFT) and loin eye area (LEA). The quality of hanwoo beef in the commercial beef market is directly influenced by the abundance of marbling. Studying the association of polymorphisms in genes involved in physiological or biological process with a quantitative trait like carcass traits can be an effective approach for developing markers for selective breeding . APM1 has been proposed as the most probable gene for obesity and also was found to have strong linkage with body mass index in humans . Genome wide association studies (GWAS) showed that - SNPs in APM1 was associated with waist circumference  and with bone metabolism in humans . Studies have reported significant effects of SNPs in the promoter of APM1 on beef carcass traits [2, 6, 12]. These results show that APM1 is associated with fat metabolism and body mass, and suggests that they may have a strong effect on carcass traits in cattle especially on MAR.
Therefore SNPs identified to have a significant association with carcass traits could be used in the national animal breeding program . Our previous reports found genetic effects of SNPs located in the APM1 gene on carcass traits in Hanwoo . In particular, the SNP g.81966377T > C revealed a significant association with carcass traits. However in order to use this SNP as a standardized molecular marker its genetic effects has to be verified on a randomly sampled population involving a significant number of samples.
The aim of this study was to verify the genetic effect of the SNP g.81966377T > C and an indel (g.81966364D > I) located in the promoter of AMP1 on carcass traits in a nationwide randomly sampled Hanwoo cattle.
Animal and carcass traits
Descriptive summaries for the measurements of carcass traits for Hanwoo cattle
MAR (1 - 9)
5.60 ± 1.95
12.64 ± 5.32
90.77 ± 10.86
428.10 ± 50.03
31.11 ± 3.56
4.11 ± 1.80
11.90 ± 5.20
80.64 ± 11.27
336.03 ± 48.69
52.54 ± 22.75
Genomic DNA isolation
Approximately 1 g of the collected tissue was used for DNA isolation using a commercial kit (Wizard DNA extraction kit, Promega) according to the manufactures guidelines. The integrity of the DNA was checked by agarose gel electrophoresis and a NanoDrop 1000 spectrophotometer (Thermo Scientific, USA). The genomic DNAs were then stored at -70 °C until further use.
The bovine APM1 gene sequence (GenBank: JQ7755868) was retrieved from the Genbank database to design PCR primers using DNA select program of the DNAstar package (Version 6.0). The forward and reverse sequences are CAGCTCGGTACTCATGGGGACAAG and GTGGGAGCTGATGGTGGTAACTGG, respectively. The primers were designed to amplify a 346 bp of the APM1 promoter region which included the targeted SNP (g.81966377T > C) and indel (g.81966364D > I) that was previously identified [2, 6, 8, 12].
The PCR reaction included 50 ng genomic DNA, 1 x reaction buffer, 2.5 mM dNTP, 10 pmoles of each primers and 1 unit Taq DNA polymerase (Genetbio, Korea) in a 20 μl reaction. The PCR condition was as follows; initial-denaturation at 95 °C for 5 min, followed by 35 cycles of denaturation at 95 °C for 45 s, annealing at 57 °C for 30 s and Extension at 72 °C for 1 min and a final extension at 72 °C for 5 mins on a thermal cycler (Veriti®96-well, Applied Biosystems, USA). The PCR product was visualized on an agarose gel stained with a fluorescent dye (Morning bio, Korea) under UV light.
The remaining PCR products were purified using a PCR purification kit (Nucleogen, Korea) for sequencing analysis with reverse primer. The sequencing reactions were carried out in 10 μl reaction containing 1 μl of purified PCR product, 2 μl of 5 x sequencing buffer, 1.6 pico moles of reverse primer and 0.5 ul Bigdye®Teminator (Applied Biosystem, USA). The sequencing PCR were carried out for 35 cycles of 94 °C for 10 s, 57 °C for 10 s, and 60 °C for 3.5 min. The amplification product was precipitated by isopropanol-ethanol precipitation and reconstituted in 10 μl of formamide. After denaturation at 95 °C for 5 min the sequences were analyzed with ABI3730 XL Genetic Analyzer (Applied Biosystems, USA) at NIAS. The resulting sequences were aligned using SeqMan program of the DNAstar Package (Version 6.0) and the genotypes were determined according to the sequence chromatogram.
After curating the data for missing phenotypes and genotypes, 8,378 samples were used for the statistical analysis.
= observed for the target trait
= overall mean of the target trait
= genotype effect
= sex effect
= the regression coefficient for age
= age (covariate)
= random error.
Least squares means were compared using Fisher’s least significant difference test with a comparison error rate of 0.005. Additive genetic effects were estimated by the difference between estimates for the two homozygous genotypes and the dominance deviation was estimated by the difference between the solution for the heterozygous genotype and the average of the solutions for the two homozygous.
Descriptive summary of carcass traits
The carcass traits of animals used in the analysis are summarized in Table 1. The traits reported are MAR, BFT, CAW, LEA and Age.
Least squares means and standard errors of marbling, backfat thickness, loineye area, and carcass weight traits according to the genotypes of APM1 genes in Hanwoo
g.81966377T > C
CC (N = 3943)
CT (N = 3610)
TT (N =825)
4.992 ± 0.03a
4.942 ± 0.03b
4.693 ± 0.04c
0.298 ± 0.06***
0.198 ± 0.08
11.43 ± 0.13c
12.335 ± 0.08b
12.558 ± 0.10a
1.127 ± 0.16***
0.681 ± 0.23
85.512 ± 0.28b
86.325 ± 0.22a
86.563 ± 0.18a
0.812 ± 0.36*
1.287 ± 0.51
390.646 ± 1.38c
392.985 ± 0.87b
393.322 ± 1.22a
2.675 ± 1.75
2.002 ± 2.48
g.81966364D > I
D/D (N = 6336)
D/I (N = 1790)
I/I (N = 252)
4.544 ± 0.02a
4.82 ± 0.5b
4.885 ± 0.13b
−0.341 ± 0.14**
0.174 ± 0.14
11.815 ± 0.07
11.992 ± 0.13
12.409 ± 0.37
−0.594 ± 0.37
−0.241 ± 0.46
84.388 ± 0.15
84.729 ± 0.29
85.518 ± 0.8
−1.129 ± 0.81
−0.448 ± 1.01
377.349 ± 0.67
377.537 ± 1.27
377.809 ± 3.43
−0.46 ± 3.5
−0.085 ± 4.32
There has been huge demand for high quality beef from 1970s with increased marbling, taste, and texture . Effective grading mechanisms were put in place by 1994 in order to have an effective measure for improvements achieved. By 2010 significant improvements were achieved, with trait measurements of 407 kg CAW, 13 mm BFT, 86 cm2 LEAS and a MAR score of 5.0. In 2013 future livestock improvement goal was announced with the target of reaching a MAR score of 6.5, 90 cm2 LEA, 13 mm BFT and 438 kg CAW for hanwoo (http://ebook.mifaff.go.kr/preview/viewer/main.php?site=2&menuno=2&previewno=1381&iframe=0&dlbt=). To achieve this goal, a marker based selection becomes imperative. Though genes having effect on polygenic traits like carcass and meat quality are still unclear, potential candidate have been identified by looking for associations between important traits and some physiological and biochemical processes [8, 15].
APM1, which is involved in lipid synthesis, fatty acid oxidation, energy homeostasis, insulin sensitivity and glucose utilization  encodes an adiponectin, and is mapped to BTA 1 near the QTL for LEA, BFT and MAR . Thus, APM1 could be a functional gene that is valuable for meat quality traits especially for fat related traits. Though the direct genetic effect of the studied SNP on the gene is still to be elucidated, variations in the promoter region could directly influence its expression thereby influencing meat quality and quantity traits. Several studies have recorded variations in the bovine APM1 promoter region [2, 6, 8, 17].
Our previous analysis reported the genetic effect of g.81966377T > C and a 67 bp insertion (g.81966364D > I) in the promoter of APM1 on meat quality  showing significant association with MAR, BFT, LEA and CAW. Thus the findings suggested that the indentified SNP and indel could be used for selection program . However in order for using this SNP as a standardized genetic marker the effect of this variant on carcass traits has to be verified and validated on a large sample set that is representative of the entire Hanwoo population. Unlike our previous study which had used samples from a specific region, in this study samples were collected from KAPE located throughout the country, in all 8,378 samples were genotyped and their association with meat quality traits were analyzed. Significant additive effects were identified for MAR, BFT and LEA. Unlike the previous study the genotypes were not found to have significant association with CAW, though the genotype effect followed a TT < CT < CC which was the same as the previous study, some minor differences in the allele frequency and genotype frequencies from previous work were also identified [6, 8] but these could be attributed to the difference in the population. As for the indel, like the previous study the indel had significant effect only for MAR. Unlike found in the previous study, the sizes of the effect of the SNP and the indel on traits were comparatively smaller, this could be due to the larger sample size or due to the different population used in this study. However the effects were significant, and since they have been validated in a large sample size, they can be used as a marker for marker assisted selection programs. In addition, previous studies have reported strong association of variation in the promoter of APM1 with growth, LEA and BFT Angus  and Chinese cattle breeds . According to the studies APM1 has been confirmed to regulate bone development, yield grade and weight traits [10, 11, 18, 19]. Our results are in agreement to these previous studies with the studied SNP (g.81966377T > C) showing significant effects on BFT and LEA in Hanwoo. The genotypes of the SNP and indel can be leveraged for selecting animals with favorable traits in Hanwoo industry. Since the effect has been validated on an unbiased randomly selected Hanwoo cattle population from the entire country, this SNP and indel is fit for being used as a robust genetic marker.
The genetic effect of the SNP (g.81966377T > C) and indel (g.81966364D > I) located in the promoter of APM1 was verified in 8,378 animals. The genotypes were found to have significant association on MAR, BFT and LEA with strong additive effects, while the indel was found to have a significant association with MAR, paving the way for their use as a robus marker for selective breeding.
This work contributes to the internal project “Cooperative Research Program for Agriculture Science and Technology Development (Number PJ010220012015)” and was supported by the National Institute of Animal Science in Rural Development Administration of Korea.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Berg AH, Combs TP, Scherer PE. ACRP30/adiponectin: an adipokine regulating glucose and lipid metabolism. Trends Endocrinol Metab. 2002;13(2):84–9.View ArticlePubMedGoogle Scholar
- Morsci NS, Schnabel RD, Taylor JF. Association analysis of adiponectin and somatostatin polymorphisms on BTA1 with growth and carcass traits in Angus cattle. Anim Genet. 2006;37(6):554–62. doi:10.1111/j.1365-2052.2006.01528.x.View ArticlePubMedGoogle Scholar
- Piñeiro R, Iglesias MJ, Gallego R, Raghay K, Eiras S, Rubio J, et al. Adiponectin is synthesized and secreted by human and murine cardiomyocytes. FEBS Lett. 2005;579(23):5163–9.View ArticlePubMedGoogle Scholar
- Scherer PE, Williams S, Fogliano M, Baldini G, Lodish HF. A novel serum protein similar to C1q, produced exclusively in adipocytes. J Biol Chem. 1995;270(45):26746–9.View ArticlePubMedGoogle Scholar
- Yokota T. Paracrine regulation of fat cell formation in bone marrow cultures via adiponectin and prostaglandins. J Clin Invest. 2002;109(10):1303–10.View ArticlePubMedPubMed CentralGoogle Scholar
- Choi Y, Davis ME, Chung H. Effects of genetic variants in the promoter region of the bovine adiponectin (ADIPOQ) gene on marbling of Hanwoo beef cattle. Meat Sci. 2015;105:57–62.View ArticlePubMedGoogle Scholar
- Lee SH, Kim UH, Dang CG, Aditi S, Kim HC, Yeon SH, et al. Strategies to multiply elite cow in Hanwoo small farm. J Embryo Transf. 2013;28(2):79–85. doi:10.12750/jet.2013.28.2.79.View ArticleGoogle Scholar
- Shin S, Chung E. Novel SNPs in the bovine ADIPOQ and PPARGC1A genes are associated with carcass traits in Hanwoo (Korean cattle). Mol Biol Rep. 2013;40(7):4651–60. doi:10.1007/s11033-013-2560-0.View ArticlePubMedGoogle Scholar
- Wu X, Cooper RS, Borecki I, Hanis C, Bray M, Lewis CE, et al. A combined analysis of genomewide linkage scans for body mass index from the National Heart, Lung, and Blood Institute Family Blood Pressure Program. Am J Hum Genet. 2002;70(5):1247–56.View ArticlePubMedPubMed CentralGoogle Scholar
- Fox CS, Heard-Costa N, Cupples LA, Dupuis J, Vasan RS, Atwood LD. Genome-wide association to body mass index and waist circumference: the Framingham Heart Study 100 K project. BMC Med Genet. 2007;8 Suppl 1:S18.Google Scholar
- Biver E, Salliot C, Combescure C, Gossec L, Hardouin P, Legroux-Gerot I, et al. Influence of adipokines and ghrelin on bone mineral density and fracture risk: a systematic review and meta-analysis. J Clin Endocrinol Metab. 2011;96(9):2703–13.View ArticlePubMedGoogle Scholar
- Zhang L, Yang M, Li C, Xu Y, Sun J, Lei C, et al. Identification and genetic effect of a variable duplication in the promoter region of the cattle ADIPOQ gene. Anim Genet. 2014;45(2):171–9. doi:10.1111/age.12112.View ArticlePubMedGoogle Scholar
- Lee SH, Park BH, Sharma A, Dang CG, Lee SS, Choi TJ, et al. Hanwoo cattle: origin, domestication, breeding strategies and genomic selection. J Anim Sci Tech. 2014;56:2. doi:10.1186/2055-0391-56-2.View ArticleGoogle Scholar
- Statistical Analysis System (SAS). SAS User's Guide, version 8. Cary, NC: Statistics SAS Institute, Inc.; 1999.Google Scholar
- Yao M-C. Programmed DNA deletions in Tetrahymena: mechanisms and implications. Trends Genet. 1996;12(1):26–30.View ArticlePubMedGoogle Scholar
- Dall’Olio S, Davoli R, Buttazzoni L, Zambonelli P, Russo V. Study of porcine adiponectin (ADIPOQ) gene and association of a missense mutation with EBVs for production and carcass traits in Italian Duroc heavy pigs. Livest Sci. 2009;125(1):101–4.View ArticleGoogle Scholar
- Zhang L, Chen H, Lan X, Zhang C, Zhang L, Zhang A, et al. The novel 5 bp deletion polymorphism in the promoter region of bovine ACRP30 gene. Mol Biol Rep. 2009;36(5):895–9.Google Scholar
- Berner HS, Lyngstadaas SP, Spahr A, Monjo M, Thommesen L, Drevon CA, et al. Adiponectin and its receptors are expressed in bone-forming cells. Bone. 2004;35(4):842–9.View ArticlePubMedGoogle Scholar
- Oshima K, Nampei A, Matsuda M, Iwaki M, Fukuhara A, Hashimoto J, et al. Adiponectin increases bone mass by suppressing osteoclast and activating osteoblast. Biochem Biophys Res Commun. 2005;331(2):520–6.View ArticlePubMedGoogle Scholar