Influence of 1α, 25-dihydroxyvitamin D3 [1, 25(OH)2D3] on the expression of Sox 9 and the transient receptor potential vanilloid 5/6 ion channels in equine articular chondrocytes
© Hdud and Loughna; licensee BioMed Central. 2014
Received: 26 June 2014
Accepted: 19 November 2014
Published: 17 December 2014
Sox 9 is a major marker of chondrocyte differentiation. When chondrocytes are cultured in vitro they progressively de-differentiate and this is associated with a decline in Sox 9 expression. The active form of vitamin D, 1, 25 (OH)2D3 has been shown to be protective of cartilage in both humans and animals. In this study equine articular chondrocytes were grown in culture and the effects of 1, 25 (OH)2D3 upon Sox 9 expression examined. The expression of the transient receptor potential vanilloid (TRPV) ion channels 5 and 6 in equine chondrocytes in vitro, we have previously shown, is inversely correlated with de-differentiation. The expression of these channels in response to 1, 25 (OH)2D3 administration was therefore also examined.
The active form of vitamin D (1, 25 (OH)2D3) when administered to cultured equine chondrocytes at two different concentrations significantly increased the expression of Sox 9 at both. In contrast 1, 25 (OH)2D3 had no significant effect upon the expression of either TRPV 5 or 6 at either the protein or the mRNA level.
The increased expression of Sox 9, in equine articular chondrocytes in vitro, in response to the active form of vitamin D suggests that this compound could be utilized to inhibit the progressive de-differentiation that is normally observed in these cells. It is also supportive of previous studies indicating that 1α, 25-dihydroxyvitamin D3 can have a protective effect upon cartilage in animals in vivo. The previously observed correlation between the degree of differentiation and the expression levels of TRPV 5/6 had suggested that these ion channels may have a direct involvement in, or be modulated by, the differentiation process in vitro. The data in the present study do not support this.
The active metabolite of vitamin D, 1, 25 (OH)2D3 has been reported to be involved in the regulation of cellular functions such as differentiation, proliferation, and the immune system . Similar to other steroids, 1, 25 (OH)2D3 exerts physiological actions at two levels by genomic and non-genomic mechanisms. The genomic mechanism exerts its effect on the cells by binding to specific receptors known as vitamin D receptors, which in turn bind to retinoid X receptors to form heterodimeric complexes . These heterodimeric complexes in turn bind to VDREs (vitamin D responsive elements) of the upstream region of the responsive gene leading to activation/suppression of the target gene. In contrast, the non-genomic effect of 1, 25 (OH)2D3 is mediated though plasma membrane receptors of the target cells which are involved in ion channel activity regulation and also activate signal transduction pathways ,. Exogenous 1, 25 (OH)2D3 in mice increased chondrocyte proliferation as well as enhancing cartilage matrix mineralization . The profound effects of vitamin D on calcium metabolism are well established and it has been reported that it can protect against cartilage loss in osteoarthritis in humans and rats .
Sox 9 (Sry-type high mobility group domain) transcription factor is expressed in articular chondrocytes, central nervous and urogenital systems ,. It is expressed in all primordial cartilage tissues during embryogenesis , and functions to induce differentiation of stem cells to chondrocytes , regulate cartilage development and phenotypic maintenance during embryonic development . Studies indicate that Sox 9 is involved in chondrocyte differentiation by regulating the expression of cartilage-specific genes such as collagen type IIα1 ,, XIα2 , aggrecan , collagen link protein , cartilage oligomeric matrix protein and Cd-rap . It has been shown that Sox 9 expression is maintained in articular chondrocytes and reduced in osteoarthritic chondrocytes .
Haploinsufficiency of Sox 9 results in campomelic dysplasia, a severe syndrome resulting in inadequate cartilage formation during development leading to severe dwarfism . In chimaeric mice, Sox 9−/− the prechondrogenic mesenchyme cells were prevented from differentiating into chondrocytes and lost their ability to express chondrocyte specific genes ,. Sox 9 expression is also significantly reduced in osteoarthritic cartilage compared to normal healthy cartilage ,.
In vitro propagation of articular chondrocytes results in de-differentiation that is characterized by gradual loss of chondrocytic phenotype and acquisition of a fibroblastic phenotype  which is associated with a rapid decline in Sox 9 expression . We have previously shown in equine chondrocytes that there is an association between the degree of de-differentiation and the expression and the transient receptor potential vanilloid (TRPV) channels 5 and 6 .
The transient receptor potential (TRP) superfamily is a non-selective cation ion channels with relative calcium selectivity. The TRPV sub-family is divided into two groups. TRPV1-4 channels are non-selective ion channels with modest permeation to calcium. This group can be activated by different stimuli such as heat/cold, chemical/mechanical stresses and binding to second messengers ,. The other group comprises of TRPV5 and TRPV6 channels that are highly calcium selective and tightly regulated by cytosolic Ca2+ concentration ,. The TRPV5 channel is implicated in Ca2+ reabsorption from the kidney, whereas TRPV6 channel is involved in Ca2+ absorption in the intestine . Expression of both channels in human articular chondrocytes at mRNA level has been reported . We also demonstrated their expression at the protein levels in equine articular chondrocytes (EAC) . A correlation between the expression of TRPV5/6 channels and administered 1, 25 (OH)2D3 concentration has also reported in intestinal endothelial cells ,, renal cells , osteoblasts ,. Calbindin-D9K is a cytosolic Ca2+ binding protein, a member of cellular proteins found in the cells with high affinity for Ca2+ ions. Calbindin-D9k knockout mice models demonstrated that 1, 25 (OH)2D3 intake increased the expression of both channels . The aim of the present study was to examine the effects of 1, 25 (OH)2D3 on the expression of Sox 9 and TRPV 5 and 6 in cultured equine chondrocytes.
Articular chondrocytes were isolated from equine articular cartilage removed from load bearing synovial joints (metacarpophalangeal joints) of skeletally mature animals obtained on the day of slaughter from a local abattoir; these animals were euthanized for purposes other than research. All experiments were performed with local institutional ethical approval (University of Nottingham, School of Veterinary Medicine and Science Ethical Committee), in strict accordance with national guidelines. Articular cartilage slices were collected in serum free DMEM medium supplemented with 2% antibiotics (50 U/ml penicillin and 50 μg/ml streptomycin) (Invitrogen, UK). Cartilage slices were washed in phosphate buffer saline (PBS) supplemented with 10% antibiotics (50 U/ml penicillin and 50 μg/ml) for 30 min with agitation, followed by enzymatic digestion in freshly prepared 0.1% (v/w) collagenase type I from clostridium histolyticum enzyme dissolved in serum free medium supplemented with 2% antibiotics at relative humidity of 95%, 5% CO2, 37°C for 18 h. Undigested cartilage debris were removed from the cell/medium suspension by filtering the mixture through a nylon filter strainer of 70 μm pore size (BD Bioscience, Europe). Supernatant was spun to isolate the chondrocytes, followed by three washes using PBS containing 10% antibiotics. Finally cells were suspended in DMEM contains 2% antibiotics and 10% FCS and cultured at 37°C, 95% humidity and 5% CO2 until confluent. Cells viability was assessed by the trypan blue exclusion test (Sigma Aldrich, UK).
Vitamin D treatment
To explore the influence of the active form of vitamin D (1, 25, α-dihydroxy vit D) on equine articular chondrocytes, cells were cultivated at 2 × 105 cells/well until sub-confluence. Chondrocytes were treated with different concentrations of 1, 25 (OH)2D3 (1 × 10−9 and 1 × 10−12)  in serum free-medium for 24 h. The concentration of DMSO was below 0.1%. At the end of 1, 25 (OH)2D3 treatment, cells were washed three times sterile PBS and whole cell lysate was collected for protein expression using western blotting.
Total cellular protein lysate was isolated using radio-immunopreceptation assay (RIPA) buffer (150 mM NaCl, 50 mM Tris–HCl, pH 7.5, 5 mM ethylene glycol tetraacetic acid (EGTA), 1% Triton, 0.5% sodium deoxycholate and 0.1% sodium dodecyl sulphate) supplemented with phosphatase and protease inhibitors cocktail (Roche Diagnostic, Mannheim, Germany) on ice. Protein concentration was quantified by the Bradford method. 25 μg of total protein lysate was mixed with sample buffer (0.5 M Tris–HCl, pH 6.8, 100% glycerol, 20% SDS, 0.5% bromophenol blue and 5% β-mercaptoethanol), separated on a 4/1-% polyacrylamide gel, then electrically transferred to PVDF membrane (Invitrogen, UK) by semi-dry apparatus (Bio-Rad, UK). Transferred proteins were blocked for non-specific binding in 5% (w/v) non-fat milk diluted in Tris base buffer saline with 0.1% tween 20 (TBS-T), followed by incubation with designated antibodies overnight. Membranes were washed five times in TBST, followed by 1 h incubation with goat anti-rabbit IgG conjugated with horseradish peroxidase (HRP) (Dako, UK) secondary antibody at room temperature. Finally, five washes were carried out for 5 min each followed by developing the membrane with the Amersham ECL western blot enhanced chemiluminescence kit (GE Healthcare, UK) and visualized by exposing to X-ray film.
The quantitative real time PCR was carried out using a LightCycler® 480 PCR System (Roche Diagnostics) using SYBR green DNA-binding fluorescent dye. 20 μl reactions were made in an optical 96-well reaction plate in triplicate and contained: a mixture of template cDNA (5 μl), sense and anti-sense gene specific primers (0.8 μl) (20 pmol), SYBR Green detection reagent and 3.4 μl RNA-free water. Reaction plate was sealed with ABI-prism optical adhesive cover, and spun at 2000 rpm for 2 min. The expression of target genes were normalised against GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) and HPRT (Hypoxanthine-guanine phosphoribosyltransferase) using comparative cycle of threshold (Ct) value method.
Data values are presented as the mean ± SEM. Each experiment was performed in triplicate. The relative expression on the graphs represents the mean of a combination of three experiments. The differences between animals were analyzed utilizing Student’s t-test followed by the Bonferroni correction. P values less than or equal to 0.05 were considered statically significant.
Results and discussion
The active form of vitamin D (1, 25 (OH)2D3) has been shown to play an important role in Ca2+ homeostasis. Intracellular Ca2+ concentration is involved in several chondrocyte functions including ECM biosynthesis. This study was designed to explore the possible influence of the active form of vitamin D (1, 25 (OH)2D3) treatment on the expression of Sox 9 and members of the TRP subfamily that are known as epithelial Ca2+ channels (TRPV5 and TRPV6 channels).
In chondrocytes, the mechanisms by which Sox 9 expression is regulated is of great interest due to the critical role played by this transcription factor in controlling the chondrocyte phenotype. The findings of the present study shows an elevation of Sox 9 protein in response to treatment with 1, 25 (OH)2D3. Isolation of chondrocytes results in their de-differentiation and loss of chondrocyte phenotype to fibroblast phenotype . Recent studies have reported changes in the pattern of expression of some proteins during the course of chondrocytes de-differentiation. Cultivation of de-differentiated chondrocytes in alginate gel results in restoring the expression of transcription factor Sox 9 and chondrocyte differentiation markers such as collagen type II and aggrecan . The current study indicated that treatment of cultured chondrocytes with 1, 25 (OH)2D3 results in restoring of the transcription factor Sox 9, which could indicate chondrocyte re-differentiation and restoration of the chondrocyte phenotype.
The physiological activities of Sox 9 protein were demonstrated by mouse genetic studies which indicated the importance of Sox 9 expression level in determining chondrocyte phenotype during development ,. These studies demonstrated that chondrocyte differentiation and cartilage development were severely affected by knock-out or knock-in of a single allele of Sox 9. Moreover, the campomelic dysplasia is a genetic disorder characterized by multiple developmental abnormalities including cartilage induced by haplo-insufficiency of Sox 9 .
In human normal articular chondrocytes, Sox 9 expression was progressively reduced by passage in monolayer cell culture . Moreover its expression is reduced in osteoarthritic chondrocytes compared to the normal articular chondrocytes  and has therefore been suggested to contribute in osteoarthritis disease processes by altering the ECM gene expression . Thus, improving the expression of Sox 9 by 1, 25 (OH)2D3 treatment could provide a new insight into the prevention and/or treatment of osteoarthritis.
In fetal cartilage as well as in mature cartilage, the main role of Sox 9 is to maintain the chondrocyte phenotype in addition to inhibition of hypertrophic chondrocyte differentiation . Therefore, down regulation of Sox 9 expression was suggested to be a precondition for hypertrophic alteration occurring in degenerative cartilage ,. As Sox 9 protein is augmented by 1, 25 (OH)2D3 treatment, this study suggests that 1, 25 (OH)2D3 treatment could be utilized to improve the ECM proteins biosynthesis by enhancing the anabolic activities of articular chondrocytes. After further passages, chondrocytes have been shown to exhibit a more pronounced de-differentiated phenotype and lower levels of Sox 9 . Further studies on the effects of 1, 25 (OH)2D3 upon these cells would be of great interest.
In the vitamin D receptor knockout mice model, the level of gene expression pattern of the TRPV6 and 5 channels in duodenum is dramatically down-regulated, in contrast no changes are observed in gene expression of other Ca2+ transporters ,. The role of vitamin D in the stimulation of Ca2+ transporters has emerged recently. Therefore, the correlation between 1, 25 (OH)2D3 treatment and Ca2+ transport proteins were investigated in several cell types. 1, 25 (OH)2D3 was demonstrated to increase the expression of TRPV6 channel, calbinding-D9k and PMCA1b genes in Caco-2 cell lines ,. This finding was not consistent with the current study, where no changes were observed on the expression of either TRPV5 or 6 channels at mRNA level or protein levels. As stated above we have previously observed an inverse correlation between TRPV 5 and 6 levels and differentiation state. The current study suggests that the changes in levels may not be directly linked to the de-differentiation process. This however, needs further study as does the mechanism by which Sox-9 is regulated by 1, 25 (OH)2D3. Ca2+ ions and Ca2+ channels have been implicated in chondrocyte metabolism ,. In vitro studies show that augmented extracellular Ca2+ promotes differentiation toward a hypertrophic phenotype, in contrast Ca2+ reduction improves ECM protein synthesis including collagen type II and aggrecan and delays hypertrophy . Therefore, investigating the effect of the 1, 25 (OH)2D3 on the expression of Ca2+ channels could be considered as a candidate for treatment of joint diseases.
The results of the current study do not, however, suggest an involvement of TRPV5/6 ion channels in the regulation of vitamin D mediated up-regulation of sox-9.
This study examined the effects of the active form of vitamin D (1, 25 (OH)2D3) upon the expression of transcription factor Sox 9 and the calcium sensitive TRPV 5 and 6 channels, in equine articular chondrocytes, in vitro. An increased expression of Sox 9, in these chondrocytes, in response to the active form of vitamin D suggests that this compound acts to inhibit the progressive de-differentiation that is normally observed in these cells. It is also supportive of previous studies indicating that 1α, 25-dihydroxyvitamin D3 can have a protective effect upon cartilage in animals in vivo. There was no effect of vitamin D (1, 25 (OH)2D3) upon the expression of either TRPV channel at either the protein or mRNA level. We have previously observed a correlation between the degree of differentiation and the expression levels of TRPV 5/6 and suggested that these ion channels may have a direct interaction with the differentiation process in vitro. The data in the present study do not support this.
- Boyan BD, Sylvia VL, Dean DD, Del Toro F, Schwartz Z: Differential regulation of growth plate chondrocytes by 1alpha,25-(OH)2D3 and 24R,25-(OH)2D3 involves cell-maturation-specific membrane-receptor-activated phospholipid metabolism. Crit Rev Oral Biol Med. 2002, 13 (2): 143-154. 10.1177/154411130201300205.View ArticlePubMedGoogle Scholar
- Tetlow LC, Smith SJ, Mawer EB, Woolley DE: Vitamin D receptors in the rheumatoid lesion: expression by chondrocytes, macrophages, and synoviocytes. Ann Rheum Dis. 1999, 58 (2): 118-121. 10.1136/ard.58.2.118.View ArticlePubMedPubMed CentralGoogle Scholar
- Lehmann B, Meurer M: Vitamin D metabolism. Dermatol Ther. 2010, 23 (1): 2-12. 10.1111/j.1529-8019.2009.01286.x.View ArticlePubMedGoogle Scholar
- Fleet JC: Genomic and proteomic approaches for probing the role of vitamin D in health. Am J Clin Nutr. 2004, 80 (6 Suppl): 1730S-1734S.PubMedGoogle Scholar
- Xue Y, Karaplis AC, Hendy GN, Goltzman D, Miao D: Exogenous 1,25-dihydroxyvitamin D3 exerts a skeletal anabolic effect and improves mineral ion homeostasis in mice that are homozygous for both the 1alpha-hydroxylase and parathyroid hormone null alleles. Endocrinology. 2006, 147 (10): 4801-4810. 10.1210/en.2006-0403.View ArticlePubMedGoogle Scholar
- Castillo EC, Hernandez-Cueto MA, Vega-Lopez MA, Lavalle C, Kouri JB, Ortiz-Navarrete V: Effects of vitamin D supplementation during the induction and progression of osteoarthritis in a rat model. Evid Based Complement Altern Med. 2012, 2012: 156563-10.1155/2012/156563.Google Scholar
- Morais Da Silva S, Hacker A, Harley V, Goodfellow P, Swain A, Lovell-Badge R: Sox9 expression during gonadal development implies a conserved role for the gene in testis differentiation in mammals and birds. Nat Genet. 1996, 14 (1): 62-68. 10.1038/ng0996-62.View ArticlePubMedGoogle Scholar
- Zhao Q, Eberspaecher H, Lefebvre V, De Crombrugghe B: Parallel expression of Sox9 and Col2a1 in cells undergoing chondrogenesis. Dev Dyn. 1997, 209 (4): 377-386. 10.1002/(SICI)1097-0177(199708)209:4<377::AID-AJA5>3.0.CO;2-F.View ArticlePubMedGoogle Scholar
- Wright E, Hargrave MR, Christiansen J, Cooper L, Kun J, Evans T, Gangadharan U, Greenfield A, Koopman P: The Sry-related gene Sox 9 is expressed during chondrogenesis in mouse embryos. Nat Genet. 1995, 9 (1): 15-20. 10.1038/ng0195-15.View ArticlePubMedGoogle Scholar
- Shintani N, Hunziker EB: Chondrogenic differentiation of bovine synovium: bone morphogenetic proteins 2 and 7 and transforming growth factor beta1 induce the formation of different types of cartilaginous tissue. Arthritis Rheum. 2007, 56 (6): 1869-1879. 10.1002/art.22701.View ArticlePubMedGoogle Scholar
- Sive JI, Baird P, Jeziorsk M, Watkins A, Hoyland JA, Freemont AJ: Expression of chondrocyte markers by cells of normal and degenerate intervertebral discs. Mol Pathol. 2002, 55 (2): 91-97. 10.1136/mp.55.2.91.View ArticlePubMedPubMed CentralGoogle Scholar
- Lefebvre V, Huang W, Harley VR, Goodfellow PN, de Crombrugghe B: SOX 9 is a potent activator of the chondrocyte-specific enhancer of the pro alpha1(II) collagen gene. Mol Cell Biol. 1997, 17 (4): 2336-2346.View ArticlePubMedPubMed CentralGoogle Scholar
- Bridgewater LC, Lefebvre V, de Crombrugghe B: Chondrocyte-specific enhancer elements in the Col11a2 gene resemble the Col2a1 tissue-specific enhancer. J Biol Chem. 1998, 273 (24): 14998-15006. 10.1074/jbc.273.24.14998.View ArticlePubMedGoogle Scholar
- Sekiya I, Tsuji K, Koopman P, Watanabe H, Yamada Y, Shinomiya K, Nifuji A, Noda M: SOX9 enhances aggrecan gene promoter/enhancer activity and is up-regulated by retinoic acid in a cartilage-derived cell line, TC6. J Biol Chem. 2000, 275 (15): 10738-10744. 10.1074/jbc.275.15.10738.View ArticlePubMedGoogle Scholar
- Kou I, Ikegawa S: SOX9-dependent and -independent transcriptional regulation of human cartilage link protein. J Biol Chem. 2004, 279 (49): 50942-50948. 10.1074/jbc.M406786200.View ArticlePubMedGoogle Scholar
- Xie WF, Zhang X, Sakano S, Lefebvre V, Sandell LJ: Trans-activation of the mouse cartilage-derived retinoic acid-sensitive protein gene by Sox 9. J Bone Miner Res. 1999, 14 (5): 757-763. 10.1359/jbmr.19126.96.36.1997.View ArticlePubMedGoogle Scholar
- Brew CJ, Clegg PD, Boot-Handford RP, Andrew JG, Hardingham T: Gene expression in human chondrocytes in late osteoarthritis is changed in both fibrillated and intact cartilage without evidence of generalised chondrocyte hypertrophy. Ann Rheum Dis. 2010, 69 (1): 234-240. 10.1136/ard.2008.097139.View ArticlePubMedGoogle Scholar
- Wagner T, Wirth J, Meyer J, Zabel B, Held M, Zimmer J, Pasantes J, Bricarelli FD, Keutel J, Hustert E, Wolf U, Tommerup N, Schempp W, Scherer G: Autosomal sex reversal and campomelic dysplasia are caused by mutations in and around the SRY-related gene SOX9. Cell. 1994, 79 (6): 1111-1120. 10.1016/0092-8674(94)90041-8.View ArticlePubMedGoogle Scholar
- Bi W, Deng JM, Zhang Z, Behringer RR, de Crombrugghe B: Sox 9 is required for cartilage formation. Nat Genet. 1999, 22 (1): 85-89. 10.1038/8792.View ArticlePubMedGoogle Scholar
- Hargus G, Kist R, Kramer J, Gerstel D, Neitz A, Scherer G, Rohwedel J: Loss of Sox 9 function results in defective chondrocyte differentiation of mouse embryonic stem cells in vitro. Int J Dev Biol. 2008, 52 (4): 323-332. 10.1387/ijdb.072490gh.View ArticlePubMedGoogle Scholar
- Cucchiarini M, Thurn T, Weimer A, Kohn D, Terwilliger EF, Madry H: Restoration of the extracellular matrix in human osteoarthritic articular cartilage by overexpression of the transcription factor SOX9. Arthritis Rheum. 2007, 56 (1): 158-167. 10.1002/art.22299.View ArticlePubMedGoogle Scholar
- Benya PD, Padilla SR, Nimni ME: Independent regulation of collagen types by chondrocytes during the loss of differentiated function in culture. Cell. 1978, 15 (4): 1313-1321. 10.1016/0092-8674(78)90056-9.View ArticlePubMedGoogle Scholar
- Tew SR, Clegg PD, Brew CJ, Redmond CM, Hardingham TE: SOX9 transduction of a human chondrocytic cell line identifies novel genes regulated in primary human chondrocytes and in osteoarthritis. Arthritis Res Ther. 2007, 9 (5): R107-10.1186/ar2311.View ArticlePubMedPubMed CentralGoogle Scholar
- Hdud IM, El-Shafei AA, Loughna P, Barrett-Jolley R, Mobasheri A: Expression of transient receptor potential vanilloid (TRPV) channels in different passages of articular chondrocytes. Int J Mol Sci. 2012, 13 (4): 4433-4445. 10.3390/ijms13044433.View ArticlePubMedPubMed CentralGoogle Scholar
- Hdud IM, Mobasheri A, Loughna PT: Effect of osmotic stress on the expression of TRPV4 and BKCa channels and possible interaction with ERK1/2 and p38 in cultured equine chondrocytes. Am J Physiol Cell Physiol. 2014, 306 (11): C1050-C1057. 10.1152/ajpcell.00287.2013.View ArticlePubMedGoogle Scholar
- Hdud IM, Mobasheri A, Loughna PT: Effects of cyclic equibiaxial mechanical stretch on alpha-BK and TRPV4 expression in equine chondrocytes. SpringerPlus. 2014, 3: 59-10.1186/2193-1801-3-59.View ArticlePubMedPubMed CentralGoogle Scholar
- Vennekens R, Hoenderop JG, Prenen J, Stuiver M, Willems PH, Droogmans G, Nilius B, Bindels RJ: Permeation and gating properties of the novel epithelial Ca(2+) channel. J Biol Chem. 2000, 275 (6): 3963-3969. 10.1074/jbc.275.6.3963.View ArticlePubMedGoogle Scholar
- Nilius B, Vennekens R, Prenen J, Hoenderop JG, Bindels RJ, Droogmans G: Whole-cell and single channel monovalent cation currents through the novel rabbit epithelial Ca2+ channel ECaC. J Physiol. 2000, 527 (Pt 2): 239-248. 10.1111/j.1469-7793.2000.00239.x.View ArticlePubMedPubMed CentralGoogle Scholar
- Hoenderop JG, Nilius B, Bindels RJ: Calcium absorption across epithelia. Physiol Rev. 2005, 85 (1): 373-422. 10.1152/physrev.00003.2004.View ArticlePubMedGoogle Scholar
- Gavenis K, Schumacher C, Schneider U, Eisfeld J, Mollenhauer J, Schmidt-Rohlfing B: Expression of ion channels of the TRP family in articular chondrocytes from osteoarthritic patients: changes between native and in vitro propagated chondrocytes. Mol Cell Biochem. 2009, 321 (1–2): 135-143. 10.1007/s11010-008-9927-x.View ArticlePubMedGoogle Scholar
- Taparia S, Fleet JC, Peng JB, Wang XD, Wood RJ: 1,25-Dihydroxyvitamin D and 25-hydroxyvitamin D–mediated regulation of TRPV6 (a putative epithelial calcium channel) mRNA expression in Caco-2 cells. Eur J Nutr. 2006, 45 (4): 196-204. 10.1007/s00394-005-0586-3.View ArticlePubMedGoogle Scholar
- Teerapornpuntakit J, Dorkkam N, Wongdee K, Krishnamra N, Charoenphandhu N: Endurance swimming stimulates transepithelial calcium transport and alters the expression of genes related to calcium absorption in the intestine of rats. Am J Physiol Endocrinol Metab. 2009, 296 (4): E775-E786. 10.1152/ajpendo.90904.2008.View ArticlePubMedGoogle Scholar
- Embark HM, Setiawan I, Poppendieck S, van de Graaf SF, Boehmer C, Palmada M, Wieder T, Gerstberger R, Cohen P, Yun CC, Bindels RJ, Lang F: Regulation of the epithelial Ca2+ channel TRPV5 by the NHE regulating factor NHERF2 and the serum and glucocorticoid inducible kinase isoforms SGK1 and SGK3 expressed in Xenopus oocytes. Cell Physiol Biochem. 2004, 14 (4–6): 203-212. 10.1159/000080329.View ArticlePubMedGoogle Scholar
- Zanello LP, Norman AW: Multiple molecular mechanisms of 1 alpha, 25(OH)2-vitamin D3 rapid modulation of three ion channel activities in osteoblasts. Bone. 2003, 33 (1): 71-79. 10.1016/S8756-3282(03)00162-5.View ArticlePubMedGoogle Scholar
- Zhang M, Wang JJ, Chen YJ: Effects of mechanical pressure on intracellular calcium release channel and cytoskeletal structure in rabbit mandibular condylar chondrocytes. Life Sci. 2006, 78 (21): 2480-2487. 10.1016/j.lfs.2005.10.043.View ArticlePubMedGoogle Scholar
- Lee GS, Jung EM, Choi KC, Oh GT, Jeung EB: Compensatory induction of the TRPV6 channel in a calbindin-D9k knockout mouse: Its regulation by 1,25-hydroxyvitamin D3. J Cell Biochem. 2009, 108 (5): 1175-1183. 10.1002/jcb.22347.View ArticlePubMedGoogle Scholar
- Fernandez-Cancio M, Audi L, Carrascosa A, Toran N, Andaluz P, Esteban C, Granada ML: Vitamin D and growth hormone regulate growth hormone/insulin-like growth factor (GH-IGF) axis gene expression in human fetal epiphyseal chondrocytes. Growth Horm IGF Res. 2009, 19 (3): 232-237. 10.1016/j.ghir.2008.10.004.View ArticlePubMedGoogle Scholar
- Kumar D, Lassar AB: The transcriptional activity of Sox9 in chondrocytes is regulated by RhoA signaling and actin polymerization. Mol Cell Biol. 2009, 29 (15): 4262-4273. 10.1128/MCB.01779-08.View ArticlePubMedPubMed CentralGoogle Scholar
- Akiyama H, Lyons JP, Mori-Akiyama Y, Yang X, Zhang R, Zhang Z, Deng JM, Taketo MM, Nakamura T, Behringer RR, McCrea PD, de Crombrugghe B: Interactions between Sox9 and beta-catenin control chondrocyte differentiation. Genes Dev. 2004, 18 (9): 1072-1087. 10.1101/gad.1171104.View ArticlePubMedPubMed CentralGoogle Scholar
- Kawakami Y, Rodriguez-Leon J, Izpisua Belmonte JC: The role of TGFbetas and Sox9 during limb chondrogenesis. Curr Opin Cell Biol. 2006, 18 (6): 723-729. 10.1016/j.ceb.2006.10.007.View ArticlePubMedGoogle Scholar
- Tew SR, Hardingham TE: Regulation of SOX9 mRNA in human articular chondrocytes involving p38 MAPK activation and mRNA stabilization. J Biol Chem. 2006, 281 (51): 39471-39479. 10.1074/jbc.M604322200.View ArticlePubMedGoogle Scholar
- Aigner T, Gebhard PM, Schmid E, Bau B, Harley V, Poschl E: SOX9 expression does not correlate with type II collagen expression in adult articular chondrocytes. Matrix Biol. 2003, 22 (4): 363-372. 10.1016/S0945-053X(03)00049-0.View ArticlePubMedGoogle Scholar
- Bi W, Huang W, Whitworth DJ, Deng JM, Zhang Z, Behringer RR, de Crombrugghe B: Haploinsufficiency of Sox9 results in defective cartilage primordia and premature skeletal mineralization. Proc Natl Acad Sci U S A. 2001, 98 (12): 6698-6703. 10.1073/pnas.111092198.View ArticlePubMedPubMed CentralGoogle Scholar
- Gebhard PM, Gehrsitz A, Bau B, Soder S, Eger W, Aigner T: Quantification of expression levels of cellular differentiation markers does not support a general shift in the cellular phenotype of osteoarthritic chondrocytes. J Orthop Res. 2003, 21 (1): 96-101. 10.1016/S0736-0266(02)00094-3.View ArticlePubMedGoogle Scholar
- Nijenhuis T, Hoenderop JG, van der Kemp AW, Bindels RJ: Localization and regulation of the epithelial Ca2+ channel TRPV6 in the kidney. J Am Soc Nephrol. 2003, 14 (11): 2731-2740. 10.1097/01.ASN.0000094081.78893.E8.View ArticlePubMedGoogle Scholar
- Song Y, Peng X, Porta A, Takanaga H, Peng JB, Hediger MA, Fleet JC, Christakos S: Calcium transporter 1 and epithelial calcium channel messenger ribonucleic acid are differentially regulated by 1, 25 dihydroxyvitamin D3 in the intestine and kidney of mice. Endocrinology. 2003, 144 (9): 3885-3894. 10.1210/en.2003-0314.View ArticlePubMedGoogle Scholar
- Haussler MR, Whitfield GK, Kaneko I, Haussler CA, Hsieh D, Hsieh JC, Jurutka PW: Molecular mechanisms of vitamin D action. Calcif Tissue Int. 2013, 92 (2): 77-98. 10.1007/s00223-012-9619-0.View ArticlePubMedGoogle Scholar
- Hoenderop JG, Muller D, Van Der Kemp AW, Hartog A, Suzuki M, Ishibashi K, Imai M, Sweep F, Willems PH, Van Os CH, Bindels RJ: Calcitriol controls the epithelial calcium channel in kidney. J Am Soc Nephrol. 2001, 12 (7): 1342-1349.PubMedGoogle Scholar
- Bouillon R, Van Cromphaut S, Carmeliet G: Intestinal calcium absorption: molecular vitamin D mediated mechanisms. J Cell Biochem. 2003, 88 (2): 332-339. 10.1002/jcb.10360.View ArticlePubMedGoogle Scholar
- Van Cromphaut SJ, Dewerchin M, Hoenderop JG, Stockmans I, Van Herck E, Kato S, Bindels RJ, Collen D, Carmeliet P, Bouillon R, Carmeliet G: Duodenal calcium absorption in vitamin D receptor-knockout mice: functional and molecular aspects. Proc Natl Acad Sci U S A. 2001, 98 (23): 13324-13329. 10.1073/pnas.231474698.View ArticlePubMedPubMed CentralGoogle Scholar
- Wood RJ, Tchack L, Taparia S: 1,25-Dihydroxyvitamin D3 increases the expression of the CaT1 epithelial calcium channel in the Caco-2 human intestinal cell line. BMC Physiol. 2001, 1: 11-10.1186/1472-6793-1-11.View ArticlePubMedPubMed CentralGoogle Scholar
- Kirsch T, Swoboda B, von der Mark K: Ascorbate independent differentiation of human chondrocytes in vitro: simultaneous expression of types I and X collagen and matrix mineralization. Differentiation. 1992, 52 (1): 89-100. 10.1111/j.1432-0436.1992.tb00503.x.View ArticlePubMedGoogle Scholar
- Bonen DK, Schmid TM: Elevated extracellular calcium concentrations induce type X collagen synthesis in chondrocyte cultures. J Cell Biol. 1991, 115 (4): 1171-1178. 10.1083/jcb.115.4.1171.View ArticlePubMedGoogle Scholar
- Koyano Y, Hejna M, Flechtenmacher J, Schmid TM, Thonar EJ, Mollenhauer J: Collagen and proteoglycan production by bovine fetal and adult chondrocytes under low levels of calcium and zinc ions. Connect Tissue Res. 1996, 34 (3): 213-225. 10.3109/03008209609000700.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.